ArticleMolecular and cellular biology2012
Continued DNA synthesis in replication checkpoint mutants leads to fork collapse.
Article in Molecular and cellular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed.
- Analyzing DNA Replication Fork Stability and Collapse Using Chromatin Fiber Analysis and the R-ODD-BLOBS Program.Computational and structural biotechnology journal · 2026Article
- Modelling DNA replication fork stability and collapse using chromatin fiber analysis and the R-ODD-BLOBS program.bioRxiv : the preprint server for biology · 2025Article
- Stn1 supports Mec1 function in protecting stalled replication forks from degradation.PLoS genetics · 2025Article
- Checkpoint-Dependent Sensitivities to Nucleoside Analogues Uncover Specific Patterns of Genomic Instability.Current issues in molecular biology · 2025Article
- Article
- Diploidy confers genomic instability inbioRxiv : the preprint server for biology · 2025Article
- The DNA Damage Repair Function of Fission Yeast CK1 Involves Targeting Arp8, a Subunit of the INO80 Chromatin Remodeling Complex.Molecular and cellular biology · 2024Article
- Impact of 1,6-hexanediol on Schizosaccharomyces pombe genome stability.G3 (Bethesda, Md.) · 2023Article
- Rad53 arrests leading and lagging strand DNA synthesis via distinct mechanisms in response to DNA replication stress.BioEssays : news and reviews in molecular, cellular and developmental biology · 2022Article
- Determinants of RPA megafoci localization to the nuclear periphery in response to replication stress.G3 (Bethesda, Md.) · 2022Article
- Cosegregation of asymmetric features during cell division.Open biology · 2021Article
- The intra-S phase checkpoint directly regulates replication elongation to preserve the integrity of stalled replisomes.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Checkpoint functions of RecQ helicases at perturbed DNA replication fork.Current genetics · 2021Review
- MonitoringBiology open · 2021Article
- A visual atlas of meiotic protein dynamics in living fission yeast.Open biology · 2021Article
- Active Replication Checkpoint Drives Genome Instability in Fission YeastMolecular and cellular biology · 2020Article
- Translesion synthesis polymerases contribute to meiotic chromosome segregation and cohesin dynamics inJournal of cell science · 2020Article
- Characterisation of unessential genes required for survival under conditions of DNA stress.Journal, genetic engineering & biotechnology · 2020Article
- Rad53 limits CMG helicase uncoupling from DNA synthesis at replication forks.Nature structural & molecular biology · 2020Article
- Checkpoint Regulation of Nuclear Tos4 Defines S Phase Arrest in Fission Yeast.G3 (Bethesda, Md.) · 2020Article
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Authors and funding
3 authors.
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Abstract
Hydroxyurea (HU) treatment activates the intra-S phase checkpoint proteins Cds1 and Mrc1 to prevent replication fork collapse. We found that prolonged DNA synthesis occurs in cds1Δ and mrc1Δ checkpoint mutants in the presence of HU and continues after release. This is coincident with increased DNA damage measured by phosphorylated histone H2A in whole cells during release. High-resolution live-cell imaging shows that mutants first accumulate extensive replication protein A (RPA) foci, followed by increased Rad52. Both DNA synthesis and RPA accumulation require the MCM helicase. We propose that a replication fork "collapse point" in HU-treated cells describes the point at which accumulated DNA damage and instability at individual forks prevent further replication. After this point, cds1Δ and mrc1Δ forks cannot complete genome replication. These observations establish replication fork collapse as a dynamic process that continues after release from HU block.
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