ArticleMolecular and cellular biology2012
The conserved Bud20 zinc finger protein is a new component of the ribosomal 60S subunit export machinery.
Article in Molecular and cellular biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 53 citations in OpenAlex.
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- A multi-parent population approach to reveal the genetic basis of plant height in maize.BMC plant biology · 2025Article
- ZNF593 regulates the cGAS-mediated innate immune response by attenuating cGAS-DNA binding.Cell death and differentiation · 2025Article
- Ribosome biogenesis in plants requires the nuclear envelope and mitochondria localized OPENER complex.Nature communications · 2025Article
- Cryo-EM structure of the AAA+ SPATA5 complex and its role in human cytoplasmic pre-60S maturation.Nature communications · 2025Article
- Highly conserved ribosome biogenesis pathways between human and yeast revealed by the MDN1-NLE1 interaction and NLE1 containing pre-60S subunits.Nucleic acids research · 2025Article
- The novel ribosome biogenesis inhibitor usnic acid blocks nucleolar pre-60S maturation.Nature communications · 2024Article
- The RNA helicase Dbp10 coordinates assembly factor association with PTC maturation during ribosome biogenesis.Nucleic acids research · 2024Article
- Visualizing the nucleoplasmic maturation of human pre-60S ribosomal particles.Cell research · 2023Article
- Article
- A single 2'-O-methylation of ribosomal RNA gates assembly of a functional ribosome.Nature structural & molecular biology · 2023Article
- Visualizing maturation factor extraction from the nascent ribosome by the AAA-ATPase Drg1.Nature structural & molecular biology · 2022Article
- Eukaryotic Ribosome assembly and Nucleocytoplasmic Transport.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Nuclear export of the pre-60S ribosomal subunit through single nuclear pores observed in real time.Nature communications · 2021Article
- Structural basis for inhibition of the AAA-ATPase Drg1 by diazaborine.Nature communications · 2021Article
- Genome Profiling for Aflatoxin BG3 (Bethesda, Md.) · 2020Article
- Structural snapshots of human pre-60S ribosomal particles before and after nuclear export.Nature communications · 2020Article
- Article
- Shaping the Nascent Ribosome: AAA-ATPases in Eukaryotic Ribosome Biogenesis.Biomolecules · 2019Review
- The L1 stalk is required for efficient export of nascent large ribosomal subunits in yeast.RNA (New York, N.Y.) · 2019Article
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
The nuclear export of the preribosomal 60S (pre-60S) subunit is coordinated with late steps in ribosome assembly. Here, we show that Bud20, a conserved C(2)H(2)-type zinc finger protein, is an unrecognized shuttling factor required for the efficient export of pre-60S subunits. Bud20 associates with late pre-60S particles in the nucleoplasm and accompanies them into the cytoplasm, where it is released through the action of the Drg1 AAA-ATPase. Cytoplasmic Bud20 is then reimported via a Kap123-dependent pathway. The deletion of Bud20 induces a strong pre-60S export defect and causes synthetic lethality when combined with mutant alleles of known pre-60S subunit export factors. The function of Bud20 in ribosome export depends on a short conserved N-terminal sequence, as we observed that mutations or the deletion of this motif impaired 60S subunit export and generated the genetic link to other pre-60S export factors. We suggest that the shuttling Bud20 is recruited to the nascent 60S subunit via its central zinc finger rRNA binding domain to facilitate the subsequent nuclear export of the preribosome employing its N-terminal extension.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.