ArticleCell transplantation2013
Chondrogenically tuned expansion enhances the cartilaginous matrix-forming capabilities of primary, adult, leporine chondrocytes.
Article in Cell transplantation, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed.
- Scaffold-Free Strategies in Dental Pulp/Dentine Tissue Engineering: Current Status and Implications for Regenerative Biological Processes.Bioengineering (Basel, Switzerland) · 2025Review
- Rejuvenation of extensively passaged human chondrocytes to engineer functional articular cartilage.Biofabrication · 2021Article
- Cartilage tissue engineering for craniofacial reconstruction.Archives of plastic surgery · 2020Article
- Adult ovine chondrocytes in expansion culture adopt progenitor cell properties that are favorable for cartilage tissue engineering.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2020Article
- Up-Regulation of Superoxide Dismutase 2 in 3D Spheroid Formation Promotes Therapeutic Potency of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells.Antioxidants (Basel, Switzerland) · 2020Article
- Overcoming Challenges in Engineering Large, Scaffold-Free Neocartilage with Functional Properties.Tissue engineering. Part A · 2018Article
- Tissue engineering potential of human dermis-isolated adult stem cells from multiple anatomical locations.PloS one · 2017Article
- Effects of passage number and post-expansion aggregate culture on tissue engineered, self-assembled neocartilage.Acta biomaterialia · 2016Article
- Cartilage immunoprivilege depends on donor source and lesion location.Acta biomaterialia · 2015Article
- Emergence of scaffold-free approaches for tissue engineering musculoskeletal cartilages.Annals of biomedical engineering · 2015Review
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- Alteration of the fibrocartilaginous nature of scaffoldless constructs formed from leporine meniscus cells and chondrocytes through manipulation of culture and processing conditions.Cells, tissues, organs · 2013Article
- Inducing articular cartilage phenotype in costochondral cells.Arthritis research & therapy · 2013Article
- Enhancing post-expansion chondrogenic potential of costochondral cells in self-assembled neocartilage.PloS one · 2013Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
When expanded through passage, chondrocytes lose their ability to produce high-quality cartilaginous matrix. This study attempts to improve the properties of constructs formed with expanded chondrocytes through alterations in the expansion protocol and the ratio of primary to expanded chondrocytes used to form cartilage constructs. A chondrogenically tuned expansion protocol provided similar monolayer growth rates as those obtained using serum-containing medium and enhanced cartilaginous properties of resultant constructs. Various ratios of primary to chondrogenically expanded chondrocytes were then self-assembled to form neocartilage. Biochemical analysis showed that constructs formed with only expanded cells had twice the GAG per wet weight and collagen II/collagen I ratio compared to constructs formed with primary chondrocytes. Biomechanically, compressive properties of constructs formed with only passaged cells matched the instantaneous modulus and exceeded the relaxation modulus of constructs formed with only primary cells. These counterintuitive results show that, by applying proper expansion and three-dimensional culture techniques, the cartilage-forming potential of adult chondrocytes expanded through passage can be enhanced over that of primary cells.
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