ArticleBone2013
Angiogenesis is required for stress fracture healing in rats.
Article in Bone, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
32 citing papers in PubMed, 46 citations in OpenAlex.
- Unraveling stress fractures: from cellular and molecular mechanisms to therapeutic approaches.Journal of orthopaedic translation · 2026Review
- The Scientific Case for Animal Models: A Perspective From Musculoskeletal Researchers.FASEB bioAdvances · 2026Article
- Single-Cell Analysis of Molecular Mechanisms in Rapid Antler Osteogenesis During Growth and Ossification Stages.International journal of molecular sciences · 2025Article
- Massage manipulation and progression of osteosarcoma, does it really correlate: a combination of prospective and retrospective cohort study.Scientific reports · 2023Observational
- VEGFA from osteoblasts is not required for lamellar bone formation following tibial loading.Bone · 2022Article
- Effect of Inter-Fragmentary Gap Size on Neovascularization During Bone Healing: A Micro-CT Imaging Study.Frontiers in bioengineering and biotechnology · 2022Article
- Understanding Reactive Oxygen Species in Bone Regeneration: A Glance at Potential Therapeutics and Bioengineering Applications.Frontiers in bioengineering and biotechnology · 2022Review
- Postnatal Osterix but not DMP1 lineage cells significantly contribute to intramembranous ossification in three preclinical models of bone injury.Frontiers in physiology · 2022Article
- Article
- Stress fractures in uncommon location: Six case reports and review of the literature.World journal of clinical cases · 2020Article
- MicroCT vascular network analysis program: Development, validation, and comparison to manufacturer software.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2020Article
- Minimally invasive laser Doppler flowmetry is suitable for serial bone perfusion measurements in mice.Bone reports · 2019Article
- VEGFA From Early Osteoblast Lineage Cells (Osterix+) Is Required in Mice for Fracture Healing.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2019Article
- Chondrocytes Promote Vascularization in Fracture Healing Through a FOXO1-Dependent Mechanism.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2019Article
- Perfluorooctylbromide nanoparticles for ultrasound imaging and drug delivery.International journal of nanomedicine · 2018Review
- Acceleration of Fracture Healing by Overexpression of Basic Fibroblast Growth Factor in the Mesenchymal Stromal Cells.Stem cells translational medicine · 2017Article
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- Article
- Repeated Laser Doppler Flowmetry Procedures in Bone Do Not Alter Perfusion, Localized Inflammation, or Gait Patterns.Proceedings of the ... National Conference on Undergraduate Research. National Conference on Undergraduate Research · 2017Article
- Development of an in vivo bone fatigue damage model using axial compression of the rabbit forelimb.Journal of biomechanics · 2016Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Although angiogenesis and osteogenesis are critically linked, the importance of angiogenesis for stress fracture healing is unknown. In this study, mechanical loading was used to create a non-displaced stress fracture in the adult rat forelimb. Fumagillin, an anti-angiogenic agent, was used as the water soluble analogue TNP-470 (25mg/kg) as well as incorporated into lipid-encapsulated α(v)β(3) integrin targeted nanoparticles (0.25mg/kg). In the first experiment, TNP-470 was administered daily for 5 days following mechanical loading, and changes in gene expression, vascularity, and woven bone formation were quantified. Although no changes in vascularity were detected 3 days after loading, treatment-related downregulation of angiogenic (Pecam1) and osteogenic (Bsp, Osx) genes was observed at this early time point. On day 7, microCT imaging of loaded limbs revealed diminished woven bone formation in treated limbs compared to vehicle treated limbs. In the second experiment, α(v)β(3) integrin targeted fumagillin nanoparticles were administered as before, albeit with a 100-fold lower dose, and changes in vascularity and woven bone formation were determined. There were no treatment-related changes in vessel count or volume 3 days after loading, although fewer angiogenic (CD105 positive) blood vessels were present in treated limbs compared to vehicle treated limbs. This result manifested on day 7 as a reduction in total vascularity, as measured by histology (vessel count) and microCT (vessel volume). Similar to the first experiment, treated limbs had diminished woven bone formation on day 7 compared to vehicle treated limbs. These results indicate that angiogenesis is required for stress fracture healing, and may have implications for inducing rapid repair of stress fractures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.