ArticleJournal of the American Association for Laboratory Animal Science : JAALAS2012
Effect of pain management on immunization efficacy in mice.
Article in Journal of the American Association for Laboratory Animal Science : JAALAS, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 32 citations in OpenAlex.
- Administration of meloxicam to improve the welfare of mice in research: a systematic review (2000 - 2020).Veterinary research communications · 2022Pooled it
- Article
- Buprenorphine extended-release (Ethiqa XR) impacts the immunological response in mice exposed to aerosolizedFrontiers in immunology · 2026Article
- Suitability of prolonged meloxicam treatment in mice seems limited due to unfavorable pharmacokinetics, side effects, and impact on home-cage behaviors.Scientific reports · 2025Article
- Evaluation of the immunoprotective power of a multiple antigenic peptide against Aah II toxin ofVaccine: X · 2024Article
- High-dose Meloxicam Provides Improved Analgesia in Female CD1 Mice: A Pharmacokinetic and Efficacy Study.Journal of the American Association for Laboratory Animal Science : JAALAS · 2023Article
- The impact of stress and anesthesia on animal models of infectious disease.Frontiers in veterinary science · 2023Review
- Serum Buprenorphine Concentrations and Behavioral Activity in Mice After a Single Subcutaneous Injection of Simbadol, Buprenorphine SR-LAB, or Standard Buprenorphine.Journal of the American Association for Laboratory Animal Science : JAALAS · 2021Article
- Article
- A Review of the Effects of Pain and Analgesia on Immune System Function and Inflammation: Relevance for Preclinical Studies.Comparative medicine · 2019Review
- Immunomodulation Associated with Sustained-release Buprenorphine in Female CD1 Mice Challenged with Ovalbumin.Journal of the American Association for Laboratory Animal Science : JAALAS · 2019Article
- Cyclooxygenase-1 and -2 Play Contrasting Roles inJournal of immunology (Baltimore, Md. : 1950) · 2018Article
- To Treat or Not to Treat: The Effects of Pain on Experimental Parameters.Comparative medicine · 2017Review
- LPS-matured CD11c+ bone marrow-derived dendritic cells can initiate autoimmune pathology with minimal injection site inflammation.Laboratory animals · 2017Article
- Antibody production in rabbits administered Freund's complete adjuvant and carprofen concurrently.Lab animal · 2016Article
- Efficacy of Sustained-Release Buprenorphine in an Experimental Laparotomy Model in Female Mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2016Article
- Pain and Laboratory Animals: Publication Practices for Better Data Reproducibility and Better Animal Welfare.PloS one · 2016Article
- Radioprotection of the brain white matter by Mn(III) n-Butoxyethylpyridylporphyrin-based superoxide dismutase mimic MnTnBuOE-2-PyP5+.Molecular cancer therapeutics · 2015Article
- Pharmacokinetics of sustained-release analgesics in mice.Journal of the American Association for Laboratory Animal Science : JAALAS · 2014Article
- Inflammatory pain may induce cognitive impairment through an interlukin-6-dependent and postsynaptic density-95-associated mechanism.Anesthesia and analgesia · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Immunization with complete Freund adjuvant (CFA) or incomplete Freund adjuvant (IFA) is commonly viewed as painful, yet rodents may not receive analgesics due to concerns that these drugs affect the desired immune responses. Here we tested the hypothesis that pain associated with immunization with CFA or IFA in mice can be relieved without compromising the effectiveness of the immune response. After subcutaneous immunization in the leg with antigen in CFA or IFA, mice were assessed for signs of pain by using behavioral tests, including unrestricted locomotion in an open field, forced running on an automated treadmill, and voluntary wheel running. Effects of the analgesics acetaminophen, meloxicam, and buprenorphine on behavioral and antibody responses were assessed after primary and secondary immunization with the model antigen ovalbumin and after repeated immunization with a limiting dose of recombinant protective antigen from Bacillus anthracis. Open field activity and the distance traveled during forced gait analysis and voluntary wheel running both decreased after immunization. Treatment with each of the analgesics normalized some but not all of these behaviors but did not decrease the mean or maximal antibody titer after primary or repeated immunization with a moderate dose of ovalbumin or after repeated immunization with a limiting dose of protective antigen. In summary, after immunization with CFA or IFA, mice showed behavioral responses suggestive of pain. Acetaminophen, meloxicam, and buprenorphine attenuated these effects without decreasing antibody responses. Therefore, the use of these analgesics for managing rodent pain associated with CFA- or IFA-containing vaccines can be encouraged.
Indexed as
Identifiers
23043810PMC3400693W67131099What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.