ArticleMolecular microbiology2012
Escherichia coli DNA polymerase III is responsible for the high level of spontaneous mutations in mutT strains.
Article in Molecular microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Mutation-biased adaptation is consequential even in large bacterial populations.Nature communications · 2026Article
- Selective Targeting and Enhanced Photodynamic Inactivation of Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2025Article
- The proofreading activity of Pfprex from Plasmodium falciparum can prevent mutagenesis of the apicoplast genome by oxidized nucleotides.Scientific reports · 2020Article
- Intrinsically Magnetic Cells: A Review on Their Natural Occurrence and Synthetic Generation.Frontiers in bioengineering and biotechnology · 2020Review
- Nucleoside Diphosphate Kinase Escalates A-to-C Mutations in MutT-Deficient Strains of Escherichia coli.Journal of bacteriology · 2019Article
- Oxygen and RNA in stress-induced mutation.Current genetics · 2018Review
- Therapeutics for Inflammatory-Related Diseases Based on Plasmon-Activated Water: A Review.International journal of molecular sciences · 2018Review
- Innovatively Therapeutic Strategy on Lung Cancer by Daily Drinking Antioxidative Plasmon-Induced Activated Water.Scientific reports · 2018Article
- Sources of spontaneous mutagenesis in bacteria.Critical reviews in biochemistry and molecular biology · 2018Review
- Replisome-mediated translesion synthesis by a cellular replicase.The Journal of biological chemistry · 2017Article
- Determinants of spontaneous mutation in the bacterium Escherichia coli as revealed by whole-genome sequencing.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Removal of 8-oxo-GTP by MutT hydrolase is not a major contributor to transcriptional fidelity.Nucleic acids research · 2014Article
- Perturbation of iron homeostasis promotes the evolution of antibiotic resistance.Molecular biology and evolution · 2014Article
- Characterization of Streptococcus tigurinus small-colony variants causing prosthetic joint infection by comparative whole-genome analyses.Journal of clinical microbiology · 2014Article
- Cell death from antibiotics without the involvement of reactive oxygen species.Science (New York, N.Y.) · 2013Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reactive oxygen species induce oxidative damage in DNA precursors, i.e. dNTPs, leading to point mutations upon incorporation. Escherichia coli mutT strains, deficient in the activity hydrolysing 8-oxo-7,8-dihydro-2'-deoxyguanosine 5'-triphosphate (8-oxo-dGTP), display more than a 100-fold higher spontaneous mutation frequency over the wild-type strain. 8-oxo-dGTP induces A to C transversions when misincorporated opposite template A. Here, we report that DNA pol III incorporates 8-oxo-dGTP ≈ 20 times more efficiently opposite template A compared with template C. Single, double or triple deletions of pol I, pol II, pol IV or pol V had modest effects on the mutT mutator phenotype. Only the deletion of all four polymerases led to a 70% reduction of the mutator phenotype. While pol III may account for nearly all 8-oxo-dGTP incorporation opposite template A, it only extends ≈ 30% of them, the remaining 70% being extended by the combined action of pol I, pol II, pol IV or pol V. The unique property of pol III, a C-family DNA polymerase present only in eubacteria, to preferentially incorporate 8-oxo-dGTP opposite template A during replication might explain the high spontaneous mutation frequency in E. coli mutT compared with the mammalian counterparts lacking the 8-oxo-dGTP hydrolysing activities.
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