ArticleThe Plant cell2012
Systems and trans-system level analysis identifies conserved iron deficiency responses in the plant lineage.
Article in The Plant cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 83 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
83 citing papers in PubMed.
- Estimation of chloroplast macromolecular complex copy numbers and subunit stoichiometries during the Chlamydomonas reinhardtii cell cycle.The Plant journal : for cell and molecular biology · 2026Article
- The Elements of Life, Photosynthesis and Genomics.Journal of molecular biology · 2025Review
- Iron-sensing and redox properties of the hemerythrin-like domains of Arabidopsis BRUTUS and BRUTUS-LIKE2 proteins.Nature communications · 2025Article
- Comprehensive Analysis ofInternational journal of molecular sciences · 2024Article
- Chlamydomonas cells transition through distinct Fe nutrition stages within 48 h of transfer to Fe-free medium.Photosynthesis research · 2024Article
- Iron rescues glucose-mediated photosynthesis repression during lipid accumulation in the green alga Chromochloris zofingiensis.Nature communications · 2024Article
- Multi-omics analysis of green lineage osmotic stress pathways unveils crucial roles of different cellular compartments.Nature communications · 2024Article
- The ascorbate biosynthesis pathway in plants is known, but there is a way to go with understanding control and functions.Journal of experimental botany · 2024Review
- Short-term acidification promotes diverse iron acquisition and conservation mechanisms in upwelling-associated phytoplankton.Nature communications · 2023Article
- Pumping iron: A multi-omics analysis of two extremophilic algae reveals iron economy management.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Optimizing Fe Nutrition for Algal Growth.Methods in molecular biology (Clifton, N.J.) · 2023Article
- The landscape of Chlamydomonas histone H3 lysine 4 methylation reveals both constant features and dynamic changes during the diurnal cycle.The Plant journal : for cell and molecular biology · 2022Article
- Genomic adaptation of the picoeukaryote Pelagomonas calceolata to iron-poor oceans revealed by a chromosome-scale genome sequence.Communications biology · 2022Article
- Comparative Phenotyping of Two Commonly UsedPlants (Basel, Switzerland) · 2022Article
- Chlamydomonas ATX1 is essential for Cu distribution to multiple cupro-enzymes and maintenance of biomass in conditions demanding cupro-enzyme-dependent metabolic pathways.Plant direct · 2022Article
- Article
- Genome-Wide Identification, Characterization and Expression Analysis of SoybeanInternational journal of molecular sciences · 2021Article
- Long-term acclimation to cadmium exposure reveals extensive phenotypic plasticity in Chlamydomonas.Plant physiology · 2021Article
- Photoprotection during iron deficiency is mediated by the bHLH transcription factors PYE and ILR3.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Iron insufficiency in floral buds impairs pollen development by disrupting tapetum function.The Plant journal : for cell and molecular biology · 2021Article
23 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on by
- Erratum issuedCORRECTION.2019
Authors and funding
11 authors.
Funding
Abstract
We surveyed the iron nutrition-responsive transcriptome of Chlamydomonas reinhardtii using RNA-Seq methodology. Presumed primary targets were identified in comparisons between visually asymptomatic iron-deficient versus iron-replete cells. This includes the known components of high-affinity iron uptake as well as candidates for distributive iron transport in C. reinhardtii. Comparison of growth-inhibited iron-limited versus iron-replete cells revealed changes in the expression of genes in chloroplastic oxidative stress response pathways, among hundreds of other genes. The output from the transcriptome was validated at multiple levels: by quantitative RT-PCR for assessing the data analysis pipeline, by quantitative proteomics for assessing the impact of changes in RNA abundance on the proteome, and by cross-species comparison for identifying conserved or universal response pathways. In addition, we assessed the functional importance of three target genes, Vitamin C 2 (VTC2), monodehydroascorbate reductase 1 (MDAR1), and conserved in the green lineage and diatoms 27 (CGLD27), by biochemistry or reverse genetics. VTC2 and MDAR1, which are key enzymes in de novo ascorbate synthesis and ascorbate recycling, respectively, are likely responsible for the 10-fold increase in ascorbate content of iron-limited cells. CGLD27/At5g67370 is a highly conserved, presumed chloroplast-localized pioneer protein and is important for growth of Arabidopsis thaliana in low iron.
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