ArticleNucleic acids research2012
Sequence-specific cleavage of the RNA strand in DNA-RNA hybrids by the fusion of ribonuclease H with a zinc finger.
Article in Nucleic acids research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 21 citations in OpenAlex.
- Y-switch: a spring-loaded synthetic gene switch for robust DNA/RNA signal amplification and detection.Nucleic acids research · 2024Article
- Assessment of Strategies for Preserving Swine Viral RNA Targets in Diagnostic Specimens.Microorganisms · 2024Review
- RHAU Peptides Specific for Parallel G-Quadruplexes: Potential Applications in Chemical Biology.Molecular biotechnology · 2023Review
- Repeat RNA expansion disorders of the nervous system: post-transcriptional mechanisms and therapeutic strategies.Critical reviews in biochemistry and molecular biology · 2021Review
- Restriction endonucleases that cleave RNA/DNA heteroduplexes bind dsDNA in A-like conformation.Nucleic acids research · 2020Article
- Improving selectivity of DNA-RNA binding zinc finger using directed evolution.BMC research notes · 2019Article
- Development of a ribonuclease containing a G4-specific binding motif for programmable RNA cleavage.Scientific reports · 2019Article
- RNase H As Gene Modifier, Driver of Evolution and Antiviral Defense.Frontiers in microbiology · 2017Review
- Relaxed specificity of prokaryotic DNA methyltransferases results in DNA site-specific modification of RNA/DNA heteroduplexes.Journal of applied genetics · 2015Article
- NPDock: a web server for protein-nucleic acid docking.Nucleic acids research · 2015Article
- Bioinformatics and computational biology in Poland.PLoS computational biology · 2013Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Ribonucleases (RNases) are valuable tools applied in the analysis of RNA sequence, structure and function. Their substrate specificity is limited to recognition of single bases or distinct secondary structures in the substrate. Currently, there are no RNases available for purely sequence-dependent fragmentation of RNA. Here, we report the development of a new enzyme that cleaves the RNA strand in DNA-RNA hybrids 5 nt from a nonanucleotide recognition sequence. The enzyme was constructed by fusing two functionally independent domains, a RNase HI, that hydrolyzes RNA in DNA-RNA hybrids in processive and sequence-independent manner, and a zinc finger that recognizes a sequence in DNA-RNA hybrids. The optimization of the fusion enzyme's specificity was guided by a structural model of the protein-substrate complex and involved a number of steps, including site-directed mutagenesis of the RNase moiety and optimization of the interdomain linker length. Methods for engineering zinc finger domains with new sequence specificities are readily available, making it feasible to acquire a library of RNases that recognize and cleave a variety of sequences, much like the commercially available assortment of restriction enzymes. Potentially, zinc finger-RNase HI fusions may, in addition to in vitro applications, be used in vivo for targeted RNA degradation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.