ArticleAmerican journal of human biology : the official journal of the Human Biology Council
A highly sensitive immunoassay for interleukin-6 in dried blood spots.
Article in American journal of human biology : the official journal of the Human Biology Council. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03878160 (Mindfulness-Based Cognitive Therapy Delivered Via Group Videoconferencing for Acute Coronary Syndrome Patients With Elevated Depression Symptoms), which is not on this map. Cited by 24 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Mindfulness-Based Cognitive Therapy Delivered Via Group Videoconferencing for Acute Coronary Syndrome Patients With Elevated Depression Symptoms
Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 48 citations in OpenAlex.
- Dried Blood Spots to Assess Cardiovascular-Kidney-Metabolic Health.Journal of the American Heart Association · 2025Pooled it
- Preliminary Effects of Mindfulness Training on Inflammatory Markers and Blood Pressure in Young Adult Survivors of Cancer: Secondary Analysis of a Pilot Randomized Controlled Trial.International journal of behavioral medicine · 2022Trial
- The prospective association between stressful life events and inflammation among adolescents with a history of early institutional rearing.Development and psychopathology · 2020Trial
- The Consequences of Foster Care Versus Institutional Care in Early Childhood on Adolescent Cardiometabolic and Immune Markers: Results From a Randomized Controlled Trial.Psychosomatic medicine · 2019Trial
- Dried blood spot compared to plasma measurements of blood-based biomarkers of brain injury in neonatal encephalopathy.Pediatric research · 2019Trial
- Dried blood spot sample extraction for metabolomics and proteomics profiling for clinical trials: a descriptive exploratory study.Scientific reports · 2026Article
- Soil-Transmitted Helminths and the Intricacies of Immunoregulation: Evidence From Amazonian Ecuador for the Importance of Considering Species-Specific Effects Within the Old Friends Hypothesis.American journal of human biology : the official journal of the Human Biology Council · 2025Article
- Longitudinal investigation of neurobiological changes across pregnancy.Communications biology · 2025Article
- Remote dried blood spot collection for inflammatory markers in older adults is feasible, reliable, and valid.Brain, behavior, and immunity · 2024Article
- Sex-specific associations between childhood trauma and adult systemic inflammation in daily life.Health psychology : official journal of the Division of Health Psychology, American Psychological Association · 2024Article
- Article
- Ecoimmunology in the field: Measuring multiple dimensions of immune function with minimally invasive, field-adapted techniques.American journal of human biology : the official journal of the Human Biology Council · 2022Review
- Household air pollution from wood-burning cookstoves and C-reactive protein among women in rural Honduras.International journal of hygiene and environmental health · 2022Article
- Synchronous Telehealth Yoga and Cognitive Processing Group Therapies for Women Veterans with Posttraumatic Stress Disorder: A Multisite Randomized Controlled Trial Adapted for COVID-19.Telemedicine journal and e-health : the official journal of the American Telemedicine Association · 2022Article
- The use of dried blood spots for characterizing children's exposure to organic environmental chemicals.Environmental research · 2021Review
- Pathogen disgust sensitivity protects against infection in a high pathogen environment.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Higher Levels of Pro-inflammatory Cytokines Are Associated With Higher Levels of Glutamate in the Anterior Cingulate Cortex in Depressed Adolescents.Frontiers in psychiatry · 2021Article
- Little to Give, Much to Gain-What Can You Do With a Dried Blood Spot?Current environmental health reports · 2020Review
- Study Protocol for Teen Inflammation Glutamate Emotion Research (TIGER).Frontiers in human neuroscience · 2020Article
- Regulation of inflammation during gestation and birth outcomes: Inflammatory cytokine balance predicts birth weight and length.American journal of human biology : the official journal of the Human Biology Council · 2019Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
objectiveInterleukin-6 (IL-6) is a pro-inflammatory cytokine that is associated with the production of C-reactive protein, the acute phase response, and chronic low-grade inflammation. In this report, we describe and validate a highly sensitive enzyme-linked immunosorbent assay (ELISA) for quantifying IL-6 at low concentrations in samples of capillary whole blood collected from a simple finger stick and dried on filter paper (dried blood spots, DBS).
methodsA commercially available ELISA for IL-6 was modified to develop a protocol for the quantification of IL-6 in DBS samples. Procedures for sample elution and incubation were optimized for precision, reliability, accuracy, and lower limit of detection using small volumes of DBS. A set of 46 matched serum/DBS samples were used to evaluate agreement between serum and DBS results.
resultsThe protocol demonstrated acceptable levels of precision, reliability, accuracy, and agreement with serum-based results. The lower limit of detection was sufficiently low to measure levels of IL-6 associated with both chronic, low-grade inflammation, and acute increases in inflammatory activity.
conclusionsThis protocol adds to the growing panel of analytes validated for quantification in DBS samples and should facilitate future research on the causes and consequences of inflammation in diverse non-clinical settings.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.