ArticleCurrent protocols in cytometry2012
Quantitative fluorescent speckle microscopy (QFSM) to measure actin dynamics.
Article in Current protocols in cytometry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 40 citations in OpenAlex.
- Integrins and tetraspanins mediate CD36-actin coupling and regulate CD36 organization and signaling.The Journal of cell biology · 2026Article
- Article
- Myosin-independent stiffness sensing by fibroblasts is regulated by the viscoelasticity of flowing actin.Communications materials · 2024Article
- Multiscale imaging and quantitative analysis of plasma membrane protein-cortical actin interplay.Biophysical journal · 2023Article
- Multiscale imaging and quantitative analysis of plasma membrane protein-cortical actin interplay.bioRxiv : the preprint server for biology · 2023Article
- Extraction of accurate cytoskeletal actin velocity distributions from noisy measurements.Nature communications · 2022Article
- Automated tracking of S. pombe spindle elongation dynamics.Journal of microscopy · 2021Article
- Live Imaging of Cytoskeletal Dynamics in EmbryonicCold Spring Harbor protocols · 2021Article
- Actin flow-dependent and -independent force transmission through integrins.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Regulation and dynamics of force transmission at individual cell-matrix adhesion bonds.Science advances · 2020Article
- Regulation of axon growth by myosin II-dependent mechanocatalysis of cofilin activity.The Journal of cell biology · 2019Article
- TheBiology open · 2019Article
- Large-scale curvature sensing by directional actin flow drives cellular migration mode switching.Nature physics · 2019Article
- Nanoscale Dynamism of Actin Enables Secretory Function in Cytolytic Cells.Current biology : CB · 2018Article
- Vimentin fibers orient traction stress.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Xenopus laevis as a model system to study cytoskeletal dynamics during axon pathfinding.Genesis (New York, N.Y. : 2000) · 2017Review
- Inducible fluorescent speckle microscopy.The Journal of cell biology · 2016Article
- Competition for actin between two distinct F-actin networks defines a bistable switch for cell polarization.Nature cell biology · 2015Article
- ERK reinforces actin polymerization to power persistent edge protrusion during motility.Science signaling · 2015Article
- Dissecting protein reaction dynamics in living cells by fluorescence recovery after photobleaching.Nature protocols · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Quantitative fluorescent speckle microscopy (QFSM) is a live-cell imaging method to analyze the dynamics of macromolecular assemblies with high spatial and temporal resolution. Its greatest successes were in the analysis of actin filament and adhesion dynamics in the context of cell migration and microtubule dynamics in interphase and the meiotic/mitotic spindle. Here, focus is on the former application to illustrate the procedures of FSM imaging and the computational image processing that extracts quantitative information from these experiments. QFSM is advantageous over other methods because it measures the movement and turnover kinetics of the actin filament (F-actin) network in living cells across the entire field of view. Experiments begin with the microinjection of fluorophore-labeled actin into cells, which generate a low ratio of fluorescently labeled to endogenously unlabeled actin monomers. Spinning disk confocal or wide-field imaging then visualizes fluorophore clusters (two to eight actin monomers) within the assembled F-actin network as speckles. QFSM software identifies and computationally tracks and utilizes the location, appearance, and disappearance of speckles to derive network flows and maps of the rate of filament assembly and disassembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.