ArticleApplied and environmental microbiology2012
3' Untranslated region-dependent degradation of the aceA mRNA, encoding the glyoxylate cycle enzyme isocitrate lyase, by RNase E/G in Corynebacterium glutamicum.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 29 citations in OpenAlex.
- Dissecting Metabolic Rewiring and Gene-Metabolite Interactions by Utilizing Untargeted Metabolomics and Single-Gene Knockouts in the Model MicroorganismJournal of the American Society for Mass Spectrometry · 2026Article
- Contribution of Uncharacterized Target Genes of MxtR/ErdR to Carbon Source Utilization by Pseudomonas putida KT2440.Microbiology spectrum · 2023Article
- Development of a novel platform for recombinant protein production inSynthetic and systems biotechnology · 2022Article
- Regulation of Ribonuclease J Expression inJournal of bacteriology · 2022Article
- Engineering the glyoxylate cycle for chemical bioproduction.Frontiers in bioengineering and biotechnology · 2022Review
- Article
- Modulation of AggR levels reveals features of virulence regulation in enteroaggregative E. coli.Communications biology · 2021Article
- Induction of glutamic acid production by copper in Corynebacterium glutamicum.Applied microbiology and biotechnology · 2021Article
- Differential evolution in 3'UTRs leads to specific gene expression in Staphylococcus.Nucleic acids research · 2020Article
- Bacterial 3'UTRs: A Useful Resource in Post-transcriptional Regulation.Frontiers in molecular biosciences · 2020Review
- The Rho-Independent Transcription Terminator for theInfection and immunity · 2019Article
- AU-Rich Long 3' Untranslated Region Regulates Gene Expression in Bacteria.Frontiers in microbiology · 2018Article
- Polynucleotide Phosphorylase, RNase E/G, and YbeY Are Involved in the Maturation of 4.5S RNA in Corynebacterium glutamicum.Journal of bacteriology · 2017Article
- Regulatory 3' Untranslated Regions of Bacterial mRNAs.Frontiers in microbiology · 2017Review
- 3'-UTR engineering to improve soluble expression and fine-tuning of activity of cascade enzymes in Escherichia coli.Scientific reports · 2016Article
- CspC regulates the expression of the glyoxylate cycle genes at stationary phase in Caulobacter.BMC genomics · 2015Article
- Mycobacterium smegmatis MSMEG_3705 encodes a selective major facilitator superfamily efflux pump with multiple roles.Current microbiology · 2015Article
- Rho and RNase play a central role in FMN riboswitch regulation in Corynebacterium glutamicum.Nucleic acids research · 2015Article
- A eukaryotic-like 3' untranslated region in Salmonella enterica hilD mRNA.Nucleic acids research · 2014Article
- Metabolic engineering of Bacillus subtilis for growth on overflow metabolites.Microbial cell factories · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously reported that the Corynebacterium glutamicum RNase E/G encoded by the rneG gene (NCgl2281) is required for the 5' maturation of 5S rRNA. In the search for the intracellular target RNAs of RNase E/G other than the 5S rRNA precursor, we detected that the amount of isocitrate lyase, an enzyme of the glyoxylate cycle, increased in rneG knockout mutant cells grown on sodium acetate as the sole carbon source. Rifampin chase experiments showed that the half-life of the aceA mRNA was about 4 times longer in the rneG knockout mutant than in the wild type. Quantitative real-time PCR analysis also confirmed that the level of aceA mRNA was approximately 3-fold higher in the rneG knockout mutant strain than in the wild type. Such differences were not observed in other mRNAs encoding enzymes involved in acetate metabolism. Analysis by 3' rapid amplification of cDNA ends suggested that RNase E/G cleaves the aceA mRNA at a single-stranded AU-rich region in the 3' untranslated region (3'-UTR). The lacZ fusion assay showed that the 3'-UTR rendered lacZ mRNA RNase E/G dependent. These findings indicate that RNase E/G is a novel regulator of the glyoxylate cycle in C. glutamicum.
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