ArticleApplied and environmental microbiology2012
Genetic diversity among Clostridium botulinum strains harboring bont/A2 and bont/A3 genes.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Clostridium botulinum serotype B: microbial genetics, toxin biosynthesis, current applications, and future prospects.Archives of microbiology · 2025Review
- The Distinctive Evolution ofFrontiers in microbiology · 2021Article
- Article
- Genomic Characterization of Newly Completed Genomes of Botulinum Neurotoxin-Producing Species from Argentina, Australia, and Africa.Genome biology and evolution · 2020Article
- Article
- Pulsotype Diversity of Clostridium botulinum Strains Containing Serotypes A and/or B Genes.Foodborne pathogens and disease · 2017Article
- Reoccurrence of botulinum neurotoxin subtype A3 inducing food-borne botulism, Slovakia, 2015.Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin · 2017Article
- Diversity of Group I and II Clostridium botulinum Strains from France Including Recently Identified Subtypes.Genome biology and evolution · 2016Article
- Genomic Epidemiology of Clostridium botulinum Isolates from Temporally Related Cases of Infant Botulism in New South Wales, Australia.Journal of clinical microbiology · 2015Article
- Genomes, neurotoxins and biology of Clostridium botulinum Group I and Group II.Research in microbiology · 2015Review
- Historical and current perspectives on Clostridium botulinum diversity.Research in microbiology · 2015Review
- First report of an infant botulism case due to Clostridium botulinum type Af.Journal of clinical microbiology · 2015Article
- Genetic characterization and comparison of Clostridium botulinum isolates from botulism cases in Japan between 2006 and 2011.Applied and environmental microbiology · 2014Article
- Comparison of assembled Clostridium botulinum A1 genomes revealed their evolutionary relationship.Genomics · 2014Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clostridium botulinum type A strains are known to be genetically diverse and widespread throughout the world. Genetic diversity studies have focused mainly on strains harboring one type A botulinum toxin gene, bont/A1, although all reported bont/A gene variants have been associated with botulism cases. Our study provides insight into the genetic diversity of C. botulinum type A strains, which contain bont/A2 (n = 42) and bont/A3 (n = 4) genes, isolated from diverse samples and geographic origins. Genetic diversity was assessed by using bont nucleotide sequencing, content analysis of the bont gene clusters, multilocus sequence typing (MLST), and pulsed-field gel electrophoresis (PFGE). Sequences of bont genes obtained in this study showed 99.9 to 100% identity with other bont/A2 or bont/A3 gene sequences available in public databases. The neurotoxin gene clusters of the subtype A2 and A3 strains analyzed in this study were similar in gene content. C. botulinum strains harboring bont/A2 and bont/A3 genes were divided into six and two MLST profiles, respectively. Four groups of strains shared a similarity of at least 95% by PFGE; the largest group included 21 out of 46 strains. The strains analyzed in this study showed relatively limited genetic diversity using either MLST or PFGE.
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