ArticleApplied and environmental microbiology2012
Functional analysis of the Lactobacillus casei BL23 sortases.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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30 citing papers in PubMed, 54 citations in OpenAlex.
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- Effect of baby food supplement on the growth and metabolite of lactic acid bacteria.BMC microbiology · 2025Article
- A Peek into the Relationship Between Probiotic Characteristics of Lactiplantibacillus plantarum Strains and Sortase A Gene Expression.Current microbiology · 2025Article
- Functional genome analysis and anti-Helicobacter pylori activity of a novel bacteriocinogenic Lactococcus sp. NH2-7C from Thai fermented pork (Nham).Scientific reports · 2023Article
- Molecular strategies for the utilisation of human milk oligosaccharides by infant gut-associated bacteria.FEMS microbiology reviews · 2023Review
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- The divergent roles of sortase in the biology of Gram-positive bacteria.Cell surface (Amsterdam, Netherlands) · 2021Review
- Antioxidant and Anti-Inflammatory Properties of Probiotic Candidate Strains Isolated during Fermentation of Agave (Microorganisms · 2021Article
- GenomeFrontiers in genetics · 2021Article
- Paraprobiotics and Postbiotics of ProbioticFrontiers in nutrition · 2020Review
- Reanalysis ofMicroorganisms · 2019Article
- Adhesion mechanisms mediated by probiotics and prebiotics and their potential impact on human health.Applied microbiology and biotechnology · 2019Review
- Role of Sortase A inFrontiers in microbiology · 2019Article
- Adherence ofFrontiers in immunology · 2019Article
- New Genes Involved in Mild Stress Response Identified by Transposon Mutagenesis inFrontiers in microbiology · 2018Article
- A Single-Plasmid Genome Editing System for Metabolic Engineering ofFrontiers in microbiology · 2018Article
- CRISPR-Cas9Applied and environmental microbiology · 2017Article
- High-Quality Draft Genome Sequence ofGenome announcements · 2017Article
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- Identification of Lactobacillus proteins with different recognition patterns between immune rabbit sera and nonimmune mice or human sera.BMC microbiology · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sortases are a class of enzymes that anchor surface proteins to the cell wall of Gram-positive bacteria. Lactobacillus casei BL23 harbors four sortase genes, two belonging to class A (srtA1 and srtA2) and two belonging to class C (srtC1 and srtC2). Class C sortases were clustered with genes encoding their putative substrates that were homologous to the SpaEFG and SpaCBA proteins that encode mucus adhesive pili in Lactobacillus rhamnosus GG. Twenty-three genes encoding putative sortase substrates were identified in the L. casei BL23 genome with unknown (35%), enzymatic (30%), or adhesion-related (35%) functions. Strains disrupted in srtA1, srtA2, srtC1, and srtC2 and an srtA1 srtA2 double mutant were constructed. The transcription of all four sortase encoding genes was detected, but only the mutation of srtA1 resulted in a decrease in bacterial surface hydrophobicity. The β-N-acetyl-glucosaminidase and cell wall proteinase activities of whole cells diminished in the srtA1 mutant and, to a greater extent, in the srtA1 srtA2 double mutant. Cell wall anchoring of the staphylococcal NucA reporter protein fused to a cell wall sorting sequence was also affected in the srtA mutants, and the percentages of adhesion to Caco-2 and HT-29 intestinal epithelial cells were reduced for the srtA1 srtA2 strain. Mutations in srtC1 or srtC2 result in an undetectable phenotype. Together, these results suggest that SrtA1 is the housekeeping sortase in L. casei BL23 and SrtA2 would carry out redundant or complementary functions that become evident when SrtA1 activity is absent.
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