ArticleInternational journal of oncology2012
Established breast cancer stem cell markers do not correlate with in vivo tumorigenicity of tumor-initiating cells.
Article in International journal of oncology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- Meta-Analysis of Tumor Stem-Like Breast Cancer Cells Using Gene Set and Network Analysis.PloS one · 2016Pooled it
- Machine learning-based development of a cytotoxicity prediction model for NK cell therapy in cancers.Cellular oncology (Dordrecht, Netherlands) · 2025Article
- Evidence that cervical cancer cells cultured as tumorspheres maintain high CD73 expression and increase their protumor characteristics through TGF-β production.Cell biochemistry and function · 2022Article
- Human Primary Breast Cancer Stem Cells Are Characterized by Epithelial-Mesenchymal Plasticity.International journal of molecular sciences · 2021Article
- Cancer stem cells and macrophages: molecular connections and future perspectives against cancer.Oncotarget · 2021Review
- Distinct phenotypes of cancer cells on tissue matrix gel.Breast cancer research : BCR · 2020Article
- Tumorigenic and Metastatic Role of CD44Cancers · 2020Article
- Mammary Stem Cells in Domestic Animals: The Role of ROS.Antioxidants (Basel, Switzerland) · 2018Review
- Are breast cancer stem cells the key to resolving clinical issues in breast cancer therapy?Gland surgery · 2017Review
- The number of tumorspheres cultured from peripheral blood is a predictor for presence of metastasis in patients with breast cancer.Oncotarget · 2016Article
- Sphere-derived tumor cells exhibit impaired metastasis by a host-mediated quiescent phenotype.Oncotarget · 2015Article
- Targeting Notch, Hedgehog, and Wnt pathways in cancer stem cells: clinical update.Nature reviews. Clinical oncology · 2015Review
- Article
- The requirement for freshly isolated human colorectal cancer (CRC) cells in isolating CRC stem cells.British journal of cancer · 2015Article
- What Tumor Dynamics Modeling Can Teach us About Exploiting the Stem-Cell View for Better Cancer Treatment.Cancer informatics · 2015Review
- Sequencing of breast cancer stem cell populations indicates a dynamic conversion between differentiation states in vivo.Breast cancer research : BCR · 2014Article
- Dynamic and influential interaction of cancer cells with normal epithelial cells in 3D culture.Cancer cell international · 2014Article
- Silencing NOTCH signaling causes growth arrest in both breast cancer stem cells and breast cancer cells.British journal of cancer · 2013Article
- The role of microRNAs in breast cancer stem cells.International journal of molecular sciences · 2013Review
- Mammosphere formation in breast carcinoma cell lines depends upon expression of E-cadherin.PloS one · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The tumor-initiating capacity of primary human breast cancer cells is maintained in vitro by culturing these cells as spheres/aggregates. Inoculation of small cell numbers derived from these non-adherent cultures leads to rapid xenograft tumor formation in mice. Accordingly, injection of more differentiated monolayer cells derived from spheres results in significantly decelerated tumor growth. For our study, two breast cancer cell lines were generated from primary tumors and cultured as mammospheres or as their adherent counterparts. We examined the in vivo tumorigenicity of these cells by injecting serial dilutions into immunodeficient mice. Inoculation of 106 cells per mouse led to rapid tumor formation, irrespective of cell line or culture conditions. However, after injection of only 103 cells, solely sphere cells were highly tumorigenic. In vitro, we investigated differentiation markers, established breast CSC markers and conducted mRNA profiling. Cytokeratin 5 and 18 were increased in both monolayer cell types, indicating a more differentiated phenotype. All cell lines were CD24(-)/CD44(+) and did not express CD133, CD326 or E-cadherin. ALDH1 activity was not detectable in any cell line. A verapamil‑sensitive Hoechst side population was present in sphere cells, but there was no correlation with tumorigenicity in vivo. mRNA profiling did not reveal upregulation of relevant transcription factors. In vitro cell cycle kinetics and in vivo tumor doubling times displayed no difference between sphere and monolayer cultures. Our data indicate that intrinsic genetic and functional markers investigated are not indicative of the in vivo tumori-genicity of putative breast tumor-initiating cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.