ArticleActa biomaterialia2013
A chondroitinase-ABC and TGF-β1 treatment regimen for enhancing the mechanical properties of tissue-engineered fibrocartilage.
Article in Acta biomaterialia, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Bioassembly of Region-Specific Fibrocartilage Microtissues to Engineer Zonally Defined Meniscal Grafts.Advanced healthcare materials · 2025Article
- Three-Dimensional Cartilage Tissue Engineering Using Placenta-Derived Extra-Embryonic Mesenchymal Stem Cells: From Isolation to Differentiation.Biomedicines · 2025Article
- siRNA Treatment Enhances Collagen Fiber Formation in Tissue-Engineered Meniscus via Transient Inhibition of Aggrecan Production.Bioengineering (Basel, Switzerland) · 2024Article
- Hyaluronic acid/chitin thermosensitive hydrogel loaded with TGF-β1 promotes meniscus repair in rabbit meniscus full-thickness tear model.Journal of orthopaedic surgery and research · 2024Article
- A cell bank paradigm for preclinical evaluation of an analogous cellular product for an allogeneic cell therapy.Biofabrication · 2024Article
- Chondroitinase ABC Treatment Improves the Organization and Mechanics of 3D Bioprinted Meniscal Tissue.ACS biomaterials science & engineering · 2023Article
- Recent research advances on corrosion mechanism and protection, and novel coating materials of magnesium alloys: a review.RSC advances · 2023Review
- Time-Dependent Anabolic Response of hMSC-Derived Cartilage Grafts to Hydrostatic Pressure.Journal of tissue engineering and regenerative medicine · 2023Article
- Non-destructive, continuous monitoring of biochemical, mechanical, and structural maturation in engineered tissue.Scientific reports · 2022Article
- First study on the effect of transforming growth factor beta 1 and insulin-like growth factor 1 on the chondrogenesis of elephant articular chondrocytes in a scaffold-based 3D culture model.Veterinary world · 2022Article
- Current Perspectives on Nucleus Pulposus Fibrosis in Disc Degeneration and Repair.International journal of molecular sciences · 2022Review
- The functionality and translatability of neocartilage constructs are improved with the combination of fluid-induced shear stress and bioactive factors.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2022Article
- Advances in biomechanical and biochemical engineering methods to stimulate meniscus tissue.American journal of translational research · 2021Review
- Engineering self-assembled neomenisci through combination of matrix augmentation and directional remodeling.Acta biomaterialia · 2020Article
- Single-cell RNA-seq analysis identifies meniscus progenitors and reveals the progression of meniscus degeneration.Annals of the rheumatic diseases · 2020Article
- Considerations for translation of tissue engineered fibrocartilage from bench to bedside.Journal of biomechanical engineering · 2018Article
- Gene expression profiles of the meniscus avascular phenotype: A guide for meniscus tissue engineering.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2018Article
- Biochemical Stimulus-Based Strategies for Meniscus Tissue Engineering and Regeneration.BioMed research international · 2018Review
- A review of in-vitro fibrocartilage tissue engineered therapies with a focus on the temporomandibular joint.Archives of oral biology · 2017Review
- Next Generation Tissue Engineering of Orthopedic Soft Tissue-to-Bone Interfaces.MRS communications · 2017Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
The development of functionally equivalent fibrocartilage remains elusive despite efforts to engineer tissues such as knee meniscus, intervertebral disc and temporomandibular joint disc. Attempts to engineer these structures often fail to create tissues with mechanical properties on a par with native tissue, resulting in constructs unsuitable for clinical applications. The objective of this study was to engineer a spectrum of biomimetic fibrocartilages representative of the distinct functional properties found in native tissues. Using the self-assembly process, different co-cultures of meniscus cells and articular chondrocytes were seeded into agarose wells and treated with the catabolic agent chondroitinase-ABC (C-ABC) and the anabolic agent transforming growth factor-β1 (TGF-β1) via a two-factor (cell ratio and bioactive treatment), full factorial study design. Application of both C-ABC and TGF-β1 resulted in a beneficial or positive increase in the collagen content of treated constructs compared to controls. Significant increases in both the collagen density and fiber diameter were also seen with this treatment, increasing these values by 32 and 15%, respectively, over control values. Mechanical testing found the combined bioactive treatment to synergistically increase the Young's modulus and ultimate tensile strength of the engineered fibrocartilages compared to controls, with values reaching the lower spectrum of those found in native tissues. Together, these data demonstrate that C-ABC and TGF-β1 interact to develop a denser collagen matrix better able to withstand tensile loading. This study highlights a way to optimize the tensile properties of engineered fibrocartilage using a biochemical and a biophysical agent together to create distinct fibrocartilages with functional properties mimicking those of native tissue.
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