ArticlePediatric research2012
Arginine and citrulline protect intestinal cell monolayer tight junctions from hypoxia-induced injury in piglets.
Article in Pediatric research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 51 citations in OpenAlex.
- Enteral L-citrulline supplementation in preterm infants is safe and effective in increasing plasma arginine and citrulline levels-a pilot randomized trial.Journal of perinatology : official journal of the California Perinatal Association · 2024Trial
- Arginine Metabolic Disruption Impairs Hair Regeneration via ROS-Mediated Inactivation of mTOR Signaling in Androgenetic Alopecia.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Heightened innate immunity may trigger chronic inflammation, fatigue and post-exertional malaise in ME/CFS.npj metabolic health and disease · 2025Article
- The impact of wild-boar-derived microbiota transplantation on piglet microbiota, metabolite profile, and gut proinflammatory cytokine production differs from sow-derived microbiota.Applied and environmental microbiology · 2025Article
- Intestinal permeability disturbances: causes, diseases and therapy.Clinical and experimental medicine · 2024Review
- Effects of Dietary l-Glutamine Supplementation on the Intestinal Function and Muscle Growth of Piglets.Life (Basel, Switzerland) · 2024Article
- Effects of supplemental citrulline on thermal and intestinal morphology parameters during heat stress and feed restriction in growing pigs.Journal of animal science · 2024Article
- Leaky Gut and the Ingredients That Help Treat It: A Review.Molecules (Basel, Switzerland) · 2023Review
- Water- and feed-based arginine impacts on gut integrity in weanling pigs.Translational animal science · 2023Article
- Nutritional Supplements to Improve Outcomes in Preterm Neonates.Clinics in perinatology · 2022Review
- Untargeted Metabolomics Sensitively Differentiates Gut Bacterial Species in Single Culture and Co-Culture Systems.ACS omega · 2022Article
- Spermine protects intestinal barrier integrity through ras-related C3 botulinum toxin substrate 1/phospholipase C-γ1 signaling pathway in piglets.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2022Article
- Dietary L-citrulline modulates the growth performance, amino acid profile, and the growth hormone/insulin-like growth factor axis in broilers exposed to high temperature.Frontiers in physiology · 2022Article
- Potential Implications of Citrulline and Quercetin on Gut Functioning of Monogastric Animals and Humans: A Comprehensive Review.Nutrients · 2021Review
- Present and Future Therapeutic Approaches to Barrier Dysfunction.Frontiers in nutrition · 2021Review
- Effects of dietary components on intestinal permeability in health and disease.American journal of physiology. Gastrointestinal and liver physiology · 2020Review
- Intestinal Permeability, Inflammation and the Role of Nutrients.Nutrients · 2020Review
- Beyond Heat Stress: Intestinal Integrity Disruption and Mechanism-Based Intervention Strategies.Nutrients · 2020Review
- The protective effects of enriched citrulline fermented milk with Lactobacillus helveticus on the intestinal epithelium integrity against Escherichia coli infection.Scientific reports · 2020Article
- Metabolic studies reveal that ruminal microbes of adult steers do not degrade rumen-protected or unprotected L-citrulline.Journal of animal science · 2020Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundArginine (Arg) is deficient in the serum of the preterm neonate and is lower in those developing intestinal ischemia. We investigated whether Arg or its precursor, citrulline (Cit), protects intestinal tight junctions (TJs) from hypoxia (HX) and determined whether inducible nitric oxide (NO) plays a role.
methodsNeonatal piglet jejunal IPEC-J2 cell monolayers were treated with Arg or Cit, reversible and irreversible NO synthetase (NOS) inhibitors, and were exposed to HX. TJs were assessed by serial measurements of transepithelial electrical resistance (TEER), flux of inulin-fluorescein isothiocyanate, and immunofluorescent staining of TJ proteins.
resultsWe found that Arg and Cit were protective against HX-related damage. At the final time point (14 h), the mean TEER ratio (TEER as compared with baseline) for Arg + HX and Cit + HX was significantly higher than that for HX alone. Both Arg and Cit were associated with decreased inulin flux across hypoxic monolayers and qualitatively preserved TJ proteins. Irreversible inhibition of NOS blocked this protective effect. Lipid peroxidation assay showed that our model did not produce oxidant injury.
conclusionArg and Cit, via a mechanism dependent on NO donation, protected intestinal epithelial integrity.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.