ArticleBiochimica et biophysica acta2013
Lipidome and proteome of lipid droplets from the methylotrophic yeast Pichia pastoris.
Article in Biochimica et biophysica acta, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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22 citing papers in PubMed, 63 citations in OpenAlex.
- Protein production dynamics and physiological adaptation of recombinant Komagataella phaffii at near-zero growth rates.Microbial cell factories · 2024Article
- Dynamics of the lipid body lipidome in the oleaginous yeast Yarrowia sp.FEMS yeast research · 2024Article
- Hif1α/Dhrs3a Pathway Participates in Lipid Droplet Accumulation via Retinol and Ppar-γ in Fish Hepatocytes.International journal of molecular sciences · 2023Article
- Lipid Composition ofFood technology and biotechnology · 2020Article
- Preparative supercritical fluid chromatography for lipid class fractionation-a novel strategy in high-resolution mass spectrometry based lipidomics.Analytical and bioanalytical chemistry · 2020Article
- A subcellular proteome atlas of the yeast Komagataella phaffii.FEMS yeast research · 2020Article
- The Puzzling Conservation and Diversification of Lipid Droplets from Bacteria to Eukaryotes.Results and problems in cell differentiation · 2020Article
- Review
- Biotechnological Production of the Cell Penetrating Antifungal PAF102 Peptide inFrontiers in microbiology · 2019Article
- Lipid Droplets: Formation to Breakdown.Lipids · 2017Review
- The effect of hypoxia on the lipidome of recombinant Pichia pastoris.Microbial cell factories · 2017Article
- Comparative Subcellular Proteomics Analysis of Susceptible and Near-isogenic Resistant Bombyx mori (Lepidoptera) Larval Midgut Response to BmNPV infection.Scientific reports · 2017Article
- The Role of Lipid Droplets in Mortierella alpina Aging Revealed by Integrative Subcellular and Whole-Cell Proteome Analysis.Scientific reports · 2017Article
- The protein and neutral lipid composition of lipid droplets isolated from the fission yeast, Schizosaccharomyces pombe.Journal of microbiology (Seoul, Korea) · 2017Article
- Biology and pathobiology of lipid droplets and their potential role in the protection of the organ of Corti.Hearing research · 2015Review
- Lipids containing medium-chain fatty acids are specific to post-whole genome duplication Saccharomycotina yeasts.BMC evolutionary biology · 2015Article
- Dynamics of the lipid droplet proteome of the Oleaginous yeast rhodosporidium toruloides.Eukaryotic cell · 2015Article
- Improving functional annotation for industrial microbes: a case study with Pichia pastoris.Trends in biotechnology · 2014Article
- Isolation of cellular lipid droplets: two purification techniques starting from yeast cells and human placentas.Journal of visualized experiments : JoVE · 2014Article
- Microorganism lipid droplets and biofuel development.BMB reports · 2013Review
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Authors and funding
7 authors at 4 institutions in 2 countries.
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Abstract
Lipid droplets (LD) are the main depot of non-polar lipids in all eukaryotic cells. In the present study we describe isolation and characterization of LD from the industrial yeast Pichia pastoris. We designed and adapted an isolation procedure which allowed us to obtain this subcellular fraction at high purity as judged by quality control using appropriate marker proteins. Components of P. pastoris LD were characterized by conventional biochemical methods of lipid and protein analysis, but also by a lipidome and proteome approach. Our results show several distinct features of LD from P. pastoris especially in comparison to Saccharomyces cerevisiae. P. pastoris LD are characterized by their high preponderance of triacylglycerols over steryl esters in the core of the organelle, the high degree of fatty acid (poly)unsaturation and the high amount of ergosterol precursors. The high phosphatidylinositol to phosphatidylserine of ~7.5 ratio on the surface membrane of LD is noteworthy. Proteome analysis revealed equipment of the organelle with a small but typical set of proteins which includes enzymes of sterol biosynthesis, fatty acid activation, phosphatidic acid synthesis and non-polar lipid hydrolysis. These results are the basis for a better understanding of P. pastoris lipid metabolism and lipid storage and may be helpful for manipulating cell biological and/or biotechnological processes in this yeast.
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