ArticleJournal of virology1990
Fusion function of the Semliki Forest virus spike is activated by proteolytic cleavage of the envelope glycoprotein precursor p62.
Article in Journal of virology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 98 citations in OpenAlex.
- Article
- Mutations at the Alphavirus E1'-E2 Interdimer Interface Have Host-Specific Phenotypes.Journal of virology · 2022Article
- Dynamics of natural product Lupenone as a potential fusion inhibitor against the spike complex of novel Semliki Forest Virus.PloS one · 2022Article
- Entry Inhibitors: Efficient Means to Block Viral Infection.The Journal of membrane biology · 2020Review
- Review
- A compendium of small molecule direct-acting and host-targeting inhibitors as therapies against alphaviruses.The Journal of antimicrobial chemotherapy · 2017Review
- Viral membrane fusion.Virology · 2015Review
- Characterization of pH-sensitive molecular switches that trigger the structural transition of vesicular stomatitis virus glycoprotein from the postfusion state toward the prefusion state.Journal of virology · 2014Article
- Mutating conserved cysteines in the alphavirus e2 glycoprotein causes virus-specific assembly defects.Journal of virology · 2012Article
- Replication of alphaviruses: a review on the entry process of alphaviruses into cells.Advances in virology · 2011Article
- A structural and functional perspective of alphavirus replication and assembly.Future microbiology · 2009Review
- Mutagenesis of varicella-zoster virus glycoprotein B: putative fusion loop residues are essential for viral replication, and the furin cleavage motif contributes to pathogenesis in skin tissue in vivo.Journal of virology · 2009Article
- Role of conserved cysteines in the alphavirus E3 protein.Journal of virology · 2009Article
- Stabilization of TM trimer interactions during activation of moloney murine leukemia virus Env.Journal of virology · 2008Article
- Entry of duck hepatitis B virus into primary duck liver and kidney cells after discovery of a fusogenic region within the large surface protein.Journal of virology · 2007Article
- Furin cleavage potentiates the membrane fusion-controlling intersubunit disulfide bond isomerization activity of leukemia virus Env.Journal of virology · 2006Article
- Time- and temperature-dependent activation of hepatitis C virus for low-pH-triggered entry.Journal of virology · 2006Article
- Resuscitating mutations in a furin cleavage-deficient mutant of the flavivirus tick-borne encephalitis virus.Journal of virology · 2005Article
- Acid-resistant bovine pestivirus requires activation for pH-triggered fusion during entry.Journal of virology · 2005Article
- A conserved histidine in the ij loop of the Semliki Forest virus E1 protein plays an important role in membrane fusion.Journal of virology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The precursor protein p62 of the prototype alphavirus Semliki Forest virus (SFV) undergoes during transport to the cell surface a proteolytic cleavage to form the mature envelope glycoprotein E2. To investigate the biological significance of this cleavage event, single amino acid substitutions were introduced at the cleavages site through mutagenesis of cDNA corresponding to the structural region of the SFV genome. The phenotypes of the cleavage site mutants were studied in BHK cells by using recombinant vaccinia virus vectors. Nonconservative substitutions completely abolished p62 cleavage. Uncleaved p62 was transported with normal kinetics to the cell surface, where it became accessible to low concentrations of exogenous trypsin. The proteolytic cleavage of envelope glycoprotein precursors has been shown to activate the membrane fusion potential of viral spikes in several virus families. Here we demonstrate that the fusion function of the SFV spike is activated by the cleavage of p62. Cleavage-deficient p62 expressed at the cell surface did not function in low-pH-triggered (pH 5.5) cell-cell membrane fusion; however, cleavage of the mutated p62 with exogenous trypsin restored the fusion function. We discuss a model for SFV assembly and fusion where p62 cleavage plays a crucial role in the stability of the multimeric association of the viral envelope glycoproteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.