ArticleCell reports2012
Two mechanisms produce mutation hotspots at DNA breaks in Escherichia coli.
Article in Cell reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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Who cites it
51 citing papers in PubMed.
- Analysis of Mutagenesis in the Escherichia coli tdk Gene: The Nucleoid Protein HNS Is Required for a Dramatic Cisplatin-Induced Hotspot.Environmental and molecular mutagenesis · 2026Article
- Identification of Hot and Cold Regions for Mutagenesis in the Escherichia coli tdk Gene: A Catalog of Mutational Sites.Environmental and molecular mutagenesis · 2025Article
- GnT Motifs Can Increase T:A→G:C Mutation Rates Over 1000-fold in Bacteria.Molecular biology and evolution · 2025Article
- Drugging evolution of antibiotic resistance at a regulatory network hub.Science advances · 2023Article
- ppGpp and RNA-polymerase backtracking guide antibiotic-induced mutable gambler cells.Molecular cell · 2023Article
- Somatic mutations during rapid clonal domestication ofEvolutionary applications · 2022Article
- Distinct Potentially Adaptive Accumulation of Truncation Mutations in Salmonella enterica serovar Typhi and Salmonella enterica serovar Paratyphi A.Microbiology spectrum · 2022Article
- Stress-Induced Mutagenesis, Gambler Cells, and Stealth Targeting Antibiotic-Induced Evolution.mBio · 2022Review
- Identification of a signature of evolutionarily conserved stress-induced mutagenesis in cancer.Frontiers in genetics · 2022Article
- Reverting to single-cell biology: The predictions of the atavism theory of cancer.Progress in biophysics and molecular biology · 2021Article
- Effect of mismatch repair on the mutational footprint of the bacterial SOS mutator activity.DNA repair · 2021Article
- Mechanisms restraining break-induced replication at two-ended DNA double-strand breaks.The EMBO journal · 2021Article
- Single-molecule live-cell imaging reveals RecB-dependent function of DNA polymerase IV in double strand break repair.Nucleic acids research · 2020Article
- TheGenes · 2020Article
- Antibiotic-Induced Mutagenesis: Under the Microscope.Frontiers in microbiology · 2020Review
- Comprehensive Outline of Whole Exome Sequencing Data Analysis Tools Available in Clinical Oncology.Cancers · 2019Review
- Hidden Markov models lead to higher resolution maps of mutation signature activity in cancer.Genome medicine · 2019Article
- Cigarette smoke exposure redirects Staphylococcus aureus to a virulence profile associated with persistent infection.Scientific reports · 2019Article
- Gamblers: An Antibiotic-Induced Evolvable Cell Subpopulation Differentiated by Reactive-Oxygen-Induced General Stress Response.Molecular cell · 2019Article
- Tools To Live By: Bacterial DNA Structures Illuminate Cancer.Trends in genetics : TIG · 2019Review
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Authors and funding
3 authors.
Funding
Abstract
Mutation hotspots and showers occur across phylogeny and profoundly influence genome evolution, yet the mechanisms that produce hotspots remain obscure. We report that DNA double-strand breaks (DSBs) provoke mutation hotspots via stress-induced mutation in Escherichia coli. With tet reporters placed 2 kb to 2 Mb (half the genome) away from an I-SceI site, RpoS/DinB-dependent mutations occur maximally within the first 2 kb and decrease logarithmically to ∼60 kb. A weak mutation tail extends to 1 Mb. Hotspotting occurs independently of I-site/tet-reporter-pair position in the genome, upstream and downstream in the replication path. RecD, which allows RecBCD DSB-exonuclease activity, is required for strong local but not long-distance hotspotting, indicating that double-strand resection and gap-filling synthesis underlie local hotspotting, and newly illuminating DSB resection in vivo. Hotspotting near DSBs opens the possibility that specific genomic regions could be targeted for mutagenesis, and could also promote concerted evolution (coincident mutations) within genes/gene clusters, an important issue in the evolution of protein functions.
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