ArticleClinical and experimental immunology2012
Bead-size directed distribution of Pseudomonas aeruginosa results in distinct inflammatory response in a mouse model of chronic lung infection.
Article in Clinical and experimental immunology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 31 citations in OpenAlex.
- Differential roles of resident lung macrophages during control of murine alveolar and airwayFrontiers in tuberculosis · 2026Article
- A Murine Model ofAmerican journal of respiratory cell and molecular biology · 2025Article
- In vivo evolution of antimicrobial resistance in a biofilm model of Pseudomonas aeruginosa lung infection.The ISME journal · 2024Article
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- Selected strategies to fight pathogenic bacteria.Journal of enzyme inhibition and medicinal chemistry · 2023Review
- Biologically Relevant Murine Models of ChronicPathogens (Basel, Switzerland) · 2023Article
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- To bead or not to bead: A review ofFrontiers in physiology · 2023Review
- Non-thermal plasma causesFrontiers in cellular and infection microbiology · 2022Article
- Chicken IgY reduces the risk ofFrontiers in microbiology · 2022Article
- Adjunctive S100A8/A9 Immunomodulation Hinders Ciprofloxacin Resistance inFrontiers in cellular and infection microbiology · 2021Article
- Development of Pseudomonas aeruginosa Biofilms in Partial-Thickness Burn Wounds Using a Sprague-Dawley Rat Model.Journal of burn care & research : official publication of the American Burn Association · 2019Article
- Immune Modulating Topical S100A8/A9 Inhibits Growth of Pseudomonas aeruginosa and Mitigates Biofilm Infection in Chronic Wounds.International journal of molecular sciences · 2017Article
- Pseudomonas aeruginosa Aggregate Formation in an Alginate Bead Model System ExhibitsApplied and environmental microbiology · 2017Article
- The phagocytic fitness of leucopatches may impact the healing of chronic wounds.Clinical and experimental immunology · 2016Article
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- Polymorphonuclear leukocytes restrict growth of Pseudomonas aeruginosa in the lungs of cystic fibrosis patients.Infection and immunity · 2014Article
- Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chronic Pseudomonas aeruginosa lung infection in cystic fibrosis (CF) patients is characterized by biofilms, tolerant to antibiotics and host responses. Instead, immune responses contribute to the tissue damage. However, this may depend on localization of infection in the upper conductive or in the peripheral respiratory zone. To study this we produced two distinct sizes of small alginate beads (SB) and large beads (LB) containing P. aeruginosa. In total, 175 BALB/c mice were infected with either SB or LB. At day 1 the quantitative bacteriology was higher in the SB group compared to the LB group (P < 0·003). For all time-points smaller biofilms were identified by Alcian blue staining in the SB group (P < 0·003). Similarly, the area of the airways in which biofilms were identified were smaller (P < 0·0001). A shift from exclusively endobronchial to both parenchymal and endobronchial localization of inflammation from day 1 to days 2/3 (P < 0·05), as well as a faster resolution of inflammation at days 5/6, was observed in the SB group (P < 0·03). Finally, both the polymorphonuclear neutrophil leucocyte (PMN) mobilizer granulocyte colony-stimulating factor (G-CSF) and chemoattractant macrophage inflammatory protein-2 (MIP-2) were increased at day 1 in the SB group (P < 0·0001). In conclusion, we have established a model enabling studies of host responses in different pulmonary zones. An effective recognition of and a more pronounced host response to infection in the peripheral zones, indicating that increased lung damage was demonstrated. Therefore, treatment of the chronic P. aeruginosa lung infection should be directed primarily at the peripheral lung zone by combined intravenous and inhalation antibiotic treatment.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.