ArticleAnalytical chemistry2012
Comprehensive lipidome profiling of isogenic primary and metastatic colon adenocarcinoma cell lines.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
61 citing papers in PubMed, 131 citations in OpenAlex.
- Plasma lipidomics profiling to identify signatures of pediatric medulloblastoma.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2026Article
- The causal effects of 1400 genetically determined human blood metabolites and metabolite ratios on the risk of gastrointestinal tumors: a Mendelian randomization study.Archives of medical science : AMS · 2026Article
- Altered levels of sphingolipid metabolites in serum of locally advanced rectal cancer patients: A pilot study.Journal of medical biochemistry · 2025Article
- Unconventional T cells in anti-cancer immunity.Frontiers in immunology · 2025Review
- Article
- Anti-Cancer Mechanisms of Diarylpentanoid MS17 (1,5-Bis(2-hydroxyphenyl)-1,4-pentadiene-3-one) in Human Colon Cancer Cells: A Proteomics Approach.International journal of molecular sciences · 2024Article
- Differential lipid analysis of oxaliplatin-sensitive and resistant HCT116 cells reveals different levels of drug-induced lipid droplet formation.Analytical and bioanalytical chemistry · 2024Article
- Regulatory roles of ACSL5 in the anti-tumor function of palmitic acid (C16:0) <em>via</em> the ERK signaling pathway.European journal of histochemistry : EJH · 2023Article
- Male-Biased Gut Microbiome and Metabolites Aggravate Colorectal Cancer Development.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- DiffN Selection of Tandem Mass Spectrometry Precursors.Analytical chemistry · 2023Article
- FACS-assisted single-cell lipidome analysis of phosphatidylcholines and sphingomyelins in cells of different lineages.Journal of lipid research · 2023Article
- Multi-omics analysis of TLCD1 as a promising biomarker in pan-cancer.Frontiers in cell and developmental biology · 2023Article
- Novel lipid antigens for NKT cells in cancer.Frontiers in immunology · 2023Review
- Reprogrammed Lipid Metabolism and the Lipid-Associated Hallmarks of Colorectal Cancer.Cancers · 2022Review
- Gut microbiota modulation: a tool for the management of colorectal cancer.Journal of translational medicine · 2022Review
- Multi-Omic Analysis to Characterize Metabolic Adaptation of theMetabolites · 2022Article
- Chemical Derivatization-Aided High Resolution Mass Spectrometry for Shotgun Lipidome Analysis.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Mass Spectrometry-Based Shotgun Lipidomics for Cancer Research.Advances in experimental medicine and biology · 2021Article
- Methods of Lipidomic Analysis: Extraction, Derivatization, Separation, and Identification of Lipids.Advances in experimental medicine and biology · 2021Article
- Evaluating a targeted multiple reaction monitoring approach to global untargeted lipidomic analyses of human plasma.Rapid communications in mass spectrometry : RCM · 2020Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
A "shotgun" lipidomics strategy consisting of sequential functional group selective chemical modification reactions coupled with high-resolution/accurate mass spectrometry and "targeted" tandem mass spectrometry (MS/MS) analysis has been developed and applied toward the comprehensive identification, characterization and quantitative analysis of changes in relative abundances of >600 individual glycerophospholipid, glycerolipid, sphingolipid and sterol lipids between a primary colorectal cancer (CRC) cell line, SW480, and its isogenic lymph node metastasized derivative, SW620. Selective chemical derivatization of glycerophosphoethanolamine and glycerophosphoserine lipids using a "fixed charge" sulfonium ion containing, d(6)-S,S'-dimethylthiobutanoylhydroxysuccinimide ester (d(6)-DMBNHS) reagent was used to eliminate the possibility of isobaric mass overlap of these species with the precursor ions of all other lipids in the crude extracts, thereby enabling their unambiguous assignment, while subsequent selective mild acid hydrolysis of plasmenyl (vinyl-ether) containing lipids using formic acid enabled these species to be readily differentiated from isobaric mass plasmanyl (alkyl-ether) containing lipids. Using this approach, statistically significant differences in the abundances of numerous lipid species previously identified as being associated with cancer progression or that play known roles as mediators in a range of physiological and pathological processes were observed between the SW480 and SW620 cells. Most notably, these included increased plasmanylcholine and triglyceride lipid levels, decreased plasmenylethanolamine lipids, decreased C-16 containing sphingomyelin and ceramide lipid levels, and a dramatic increase in the abundances of total cholesterol ester and triglyceride lipids in the SW620 cells compared to those in the SW480 cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.