ArticleInvestigative ophthalmology & visual science2012
Effects of VIP on corneal reconstitution and homeostasis following Pseudomonas aeruginosa induced keratitis.
Article in Investigative ophthalmology & visual science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 15 citations in OpenAlex.
- Immunomodulatory Role of Neuropeptides in the Cornea.Biomedicines · 2022Review
- Mucosal immunology of the ocular surface.Mucosal immunology · 2022Review
- Pseudomonas aeruginosa-induced nociceptor activation increases susceptibility to infection.PLoS pathogens · 2021Article
- VIP modulates the ALX/FPR2 receptor axis toward inflammation resolution in a mouse model of bacterial keratitis.Prostaglandins & other lipid mediators · 2019Review
- Immunoregulatory role of 15-lipoxygenase in the pathogenesis of bacterial keratitis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2018Article
- Role of vasoactive intestinal peptide inInternational journal of ophthalmology · 2018Article
- VIP protects human retinal microvascular endothelial cells against high glucose-induced increases in TNF-α and enhances RvD1.Prostaglandins & other lipid mediators · 2016Article
- Extracellular matrix metalloproteinase inducer enhances host resistance against pseudomonas aeruginosa infection through MAPK signaling pathway.American journal of translational research · 2016Article
- Effect of vasoactive intestinal peptide on the wound healing of alkali-burned corneas.International journal of ophthalmology · 2016Article
- Efficacy of VIP as Treatment for Bacteria-Induced Keratitis Against Multiple Pseudomonas aeruginosa Strains.Investigative ophthalmology & visual science · 2015Article
- Non-antibiotic management of bacterial keratitis.Saudi journal of ophthalmology : official journal of the Saudi Ophthalmological SocietyArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
purposeStudies from our laboratory have demonstrated that vasoactive intestinal peptide (VIP) directly converts the normally susceptible C57BL/6J (B6) mouse to resistant after ocular infection through modulation of the inflammatory response. This study examines mechanisms by which VIP influences the healing phase following infection--specifically reconstitution of the extracellular matrix (ECM).
methodsB6 mice received daily intraperitoneal (IP) injections of VIP, while control mice were similarly injected with sterile phosphate buffered saline (PBS). Real-time RT-PCR, ELISA, and immunofluorescent staining were used to assess the effects of VIP treatment on ECM molecule expression after Pseudomonas aeruginosa-induced keratitis. We also compared the effect of VIP treatment on lipopolysaccharide (LPS)-stimulated B6- and BALB/c-derived fibroblasts.
resultsIn vivo analyses revealed that VIP treatment of P. aeruginosa-infected B6 corneas led to a significant increase in ECM molecules associated with healing/homeostasis, while those associated with ECM degradation were significantly down-regulated when compared to wild-type (WT) controls. In vitro studies revealed that VIP treatment of lipopolysaccharide-stimulated fibroblasts derived from susceptible B6 and resistant BALB/c mice expressed distinct differences in ECM molecule expression, whereby the latter expressed higher levels of ECM molecules aimed at reconstitution. Furthermore, differential expression of VIP receptor-1/VIP receptor-2 (VIPR1/VIPR2) was observed between B6 and BALB/c after VIP treatment of LPS-stimulated fibroblasts.
conclusionsVIP treatment functions to enhance ECM reconstitution, which appears to be carried out in large part by fibroblasts via VIPR2. Overall, the data from this study suggest that VIP not only regulates disease pathogenesis, but also functions to restore integrity of the corneal stroma.
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Registered trials
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