ArticleExperimental and molecular pathology2012
Characterization of human IgG repertoires in an acute HIV-1 infection.
Article in Experimental and molecular pathology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Review
- Applications of High-Throughput DNA Sequencing to Single-Domain Antibody Discovery and Engineering.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Characterization of human IgM and IgG repertoires in individuals with chronic HIV-1 infection.Virologica Sinica · 2022Article
- Review
- Phage display peptide libraries: deviations from randomness and correctives.Nucleic acids research · 2018Article
- 5' Rapid Amplification of cDNA Ends and Illumina MiSeq Reveals B Cell Receptor Features in Healthy Adults, Adults With Chronic HIV-1 Infection, Cord Blood, and Humanized Mice.Frontiers in immunology · 2018Article
- Antibody-virus co-evolution in HIV infection: paths for HIV vaccine development.Immunological reviews · 2017Review
- Antigenic landscape of the HIV-1 envelope and new immunological concepts defined by HIV-1 broadly neutralizing antibodies.Current opinion in immunology · 2016Review
- The Diversity and Molecular Evolution of B-Cell Receptors during Infection.Molecular biology and evolution · 2016Review
- High-throughput sequencing enhanced phage display enables the identification of patient-specific epitope motifs in serum.Scientific reports · 2015Article
- Antibody germline characterization of cross-neutralizing human IgGs against 4 serotypes of dengue virus.Biochemical and biophysical research communications · 2014Article
- Profiling the IgOme: meeting the challenge.FEBS letters · 2014Review
- Deep sequencing and Circos analyses of antibody libraries reveal antigen-driven selection of Ig VH genes during HIV-1 infection.Experimental and molecular pathology · 2013Article
- The application of next generation sequencing to the understanding of antibody repertoires.Frontiers in immunology · 2013Review
- Convergent antibody signatures in human dengue.Cell host & microbe · 2013Article
- Human monoclonal antibodies as candidate therapeutics against emerging viruses and HIV-1.Virologica Sinica · 2013Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
All known broadly neutralizing antibodies (bnAbs) are highly somatically mutated and therefore significantly differ from their germline predecessors. Thus although the mature bnAbs bind to conserved epitopes of the HIV-1 envelope glycoprotein (Env) with high affinity their germline predecessors do not or weakly bind Envs failing to initiate an effective immune response. The identification of less somatically mutated bnAbs and/or antibody maturation intermediates that are clonally related to bnAbs may be useful to circumvent the major problem of initiating immune responses leading to elicitation of bnAbs. Here, we describe the identification of IgG antibodies from an acutely HIV-1-infected patient using a combination of phage display and high-throughput sequencing. We found two antibodies with only a single point mutation in the V region of their heavy chain variable domains compared to their putative germline predecessors which bound with high affinity to several Envs. They targeted the Env gp41 and did not neutralize HIV-1. Using high-throughput sequencing, we identified several highly abundant CDR3s, germline-like as well as somatically mutated V genes in the VH/VL repertoires of the patient which may provide antibody intermediates corresponding to known bnAbs as templates for design of novel HIV-1 vaccine immunogens.
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