ArticleJournal of clinical microbiology2012
Novel interpretation of molecular diagnosis of congenital toxoplasmosis according to gestational age at the time of maternal infection.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 62 citations in OpenAlex.
- Pooled it
- An RPA-CRISPR/Cas12a-based rapid and sensitive nucleic acid method for detection ofMicrobiology spectrum · 2026Article
- Multicenter performance evaluation of the "quanty TOXO (RH region)" kit (Clonit) for molecular diagnosis of toxoplasmosis.Journal of clinical microbiology · 2025Article
- Molecular diagnosis of human toxoplasmosis: the state of the art.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2024Review
- Exploring the potential of Toxoplasma gondii in drug development and as a delivery system.Experimental & molecular medicine · 2024Review
- Systematic Review and Meta-Analysis of Congenital Toxoplasmosis Diagnosis: Advances and Challenges.Journal of tropical medicine · 2024Review
- Toxoplasma gondii Infection in Pregnancy - Recommendations of the Working Group on Obstetrics and Prenatal Medicine (AGG - Section on Maternal Disorders).Geburtshilfe und Frauenheilkunde · 2023Article
- Diagnostic accuracy of adjusted low IgG avidity index to predict acute Toxoplasma gondii infection in the first trimester of pregnancy.Folia parasitologica · 2022Article
- Diagnosis of Congenital Toxoplasmosis: No Benefit of IgA Antibody Detection by Platelia ELISA in a Tricentric Evaluation.Journal of clinical microbiology · 2022Article
- Impact of pre-analytic step duration on molecular diagnosis of toxoplasmosis for five types of biological samples.PloS one · 2021Article
- Inhibition of polymerase chain reaction: Pathogen-specific controls are better than human gene amplification.PloS one · 2019Article
- Evaluation ofAdvanced biomedical research · 2018Article
- Article
- Molecular diagnosis of Toxoplasma gondii infection in Libya.BMC infectious diseases · 2016Article
- Molecular diagnosis of toxoplasmosis in immunocompromised patients: a 3-year multicenter retrospective study.Journal of clinical microbiology · 2015Article
- Contribution of neonatal amniotic fluid testing to diagnosis of congenital toxoplasmosis.Journal of clinical microbiology · 2015Article
- A 10-year retrospective comparison of two target sequences, REP-529 and B1, for Toxoplasma gondii detection by quantitative PCR.Journal of clinical microbiology · 2015Article
- Multicentric comparative assessment of the bio-evolution Toxoplasma gondii detection kit with eight laboratory-developed PCR assays for molecular diagnosis of congenital toxoplasmosis.Journal of clinical microbiology · 2015Article
- Characterization and multicentric validation of a common standard for Toxoplasma gondii detection using nucleic acid amplification assays.Journal of clinical microbiology · 2014Article
- Assessment of laboratory methods used in the diagnosis of congenital toxoplasmosis after maternal treatment with spiramycin in pregnancy.BMC infectious diseases · 2014Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
From a prospective cohort of 344 women who seroconverted for toxoplasmosis during pregnancy, 344 amniotic fluid, 264 placenta, and 216 cord blood samples were tested for diagnosis of congenital toxoplasmosis using the same PCR assay. The sensitivity and negative predictive value of the PCR assay using amniotic fluid were 86.3% and 97.2%, respectively, and both specificity and positive predictive value were 100%. Using placenta and cord blood, sensitivities were 79.5% and 21.2%, and specificities were 92% and 100%, respectively. In addition, the calculation of pretest and posttest probabilities and the use of logistic regression allowed us to obtain curves that give a dynamic interpretation of the risk of congenital toxoplasmosis according to gestational age at maternal infection, as represented by the three sample types (amniotic fluid, placenta, and cord blood). Two examples are cited here: for a maternal infection at 25 weeks of amenorrhea, a negative result of prenatal diagnosis allowed estimation of the probability of congenital toxoplasmosis at 5% instead of an a priori (pretest) risk estimate of 33%. For an infection at 10 weeks of amenorrhea associated with a pretest congenital toxoplasmosis risk of 7%, a positive PCR result using placenta at birth yields a risk increase to 43%, while a negative result damps down the risk to 0.02%. Thus, with a molecular diagnosis performing at a high level, and in spite of the persistence of false negatives, posttest risk curves using both negative and positive results prove highly informative, allowing a better assessment of the actual risk of congenital toxoplasmosis and finally an improved decision guide to treatment.
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