ArticleJournal of clinical microbiology2012
Use of clustered regularly interspaced short palindromic repeat sequence polymorphisms for specific detection of enterohemorrhagic Escherichia coli strains of serotypes O26:H11, O45:H2, O103:H2, O111:H8, O121:H19, O145:H28, and O157:H7 by real-time PCR.
Article in Journal of clinical microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- TheEcoSal Plus · 2025Review
- Enhancing detection of STEC in the meat industry: insights into virulence of priority STEC.Frontiers in microbiology · 2025Article
- Molecular and in silico analyses for detection of Shiga toxin-producing Escherichia coli (STEC) and highly pathogenic enterohemorrhagic Escherichia coli (EHEC) using genetic markers located on plasmid, O Island 57 and O Island 71.BMC veterinary research · 2024Article
- Large-Scale Phylogenetic Analysis Reveals a New Genetic Clade among Escherichia coli O26 Strains.Microbiology spectrum · 2022Article
- Distribution of CRISPR inAnimals : an open access journal from MDPI · 2022Article
- Review of CRISPR-Cas Systems inInternational journal of microbiology · 2022Review
- Utilization of Clustered Regularly Interspaced Short Palindromic Repeats to GenotypeFrontiers in microbiology · 2020Article
- Prevalence of Enteropathogens and Virulence Traits in Brazilian Children With and Without Diarrhea.Frontiers in cellular and infection microbiology · 2020Article
- Clinical and molecular characteristics of carbapenem non-susceptible Escherichia coli: A nationwide survey from Oman.PloS one · 2020Article
- Mechanisms of Type I-E and I-F CRISPR-Cas Systems inEcoSal Plus · 2019Review
- Laboratory Methods in Molecular Epidemiology: Bacterial Infections.Microbiology spectrum · 2018Review
- Variability in CharacterizingMicroorganisms · 2018Article
- Genetic Analysis of Virulence Potential ofFrontiers in microbiology · 2018Article
- Pathogenomics of Virulence Traits ofFrontiers in microbiology · 2018Article
- Identification of Novel Biomarkers for Priority Serotypes of Shiga Toxin-ProducingFrontiers in microbiology · 2018Article
- Suppressing the CRISPR/Cas adaptive immune system in bacterial infections.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2017Review
- Change in the Structure ofFrontiers in microbiology · 2017Article
- The Mobilome; A Major Contributor toFrontiers in microbiology · 2017Article
- CRISPR1 analysis of naturalized surface water and fecal Escherichia coli suggests common origin.MicrobiologyOpen · 2016Article
- Genomic comparison of Escherichia coli serotype O103:H2 isolates with and without verotoxin genes: implications for risk assessment of strains commonly found in ruminant reservoirs.Infection ecology & epidemiology · 2016Article
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3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We explored the genetic diversity of the clustered regularly interspaced short palindromic repeat (CRISPR) regions of enterohemorrhagic Escherichia coli (EHEC) to design simplex real-time PCR assays for each of the seven most important EHEC serotypes worldwide. A panel of 958 E. coli strains investigated for their CRISPR loci by high-throughput real-time PCR showed that CRISPR polymorphisms in E. coli strongly correlated with both O:H serotypes and the presence of EHEC virulence factors (stx and eae genes). The CRISPR sequences chosen for simplex real-time PCR amplification of EHEC strains belonging to the top 7 EHEC serogroups differentiated clearly between EHEC and non-EHEC strains. Specificity estimates for the CRISPR PCR assays varied from 97.5% to 100%. Sensitivity estimates for the assays ranged from 95.7% to 100%. The assays targeting EHEC O145:H28, O103:H2, and O45:H2 displayed 100% sensitivity. The combined usage of two simplex PCR assays targeting different sequences of the O26 CRISPR locus allowed detection of EHEC O26:H11 with 100% sensitivity. By combining two simplex PCR assays targeting different sequences of the EHEC O157 CRISPR locus, EHEC O157:H7 was detected with 99.56% sensitivity. EHEC O111:H8 and EHEC O121:H19 were detected with 95.9% and 95.7% sensitivity, respectively. This study demonstrates that the identification of EHEC serotype-specific CRISPR sequences is more specific than the mere identification of O-antigen gene sequences, as is used in current PCR protocols for detection of EHEC strains.
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