ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2012
Optimization of a GCaMP calcium indicator for neural activity imaging.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 730 papers.
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Who cites it
730 citing papers in PubMed.
- A framework for analyzing globaleLife · 2026Article
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- Spike inference from calcium imaging data acquired with GCaMP8 indicators.Nature methods · 2026Article
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- Evidence that interglomerular inhibition generates non-monotonic concentration-response relationships in mitral/tufted glomeruli in the mouse olfactory bulb.The Journal of physiology · 2026Article
- Neuronal ensembles in cortical function and disease.Physiological reviews · 2026Review
- Precise calcium-to-spike inference using biophysical generative models.bioRxiv : the preprint server for biology · 2026Article
- A Central Somatic Transmission Mediates Proprioceptive Facilitation of Muscle Pain.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Redesign of Calmodulin for Genetically Encoded ZnChemical & biomedical imaging · 2026Article
- An Open-Source Pipeline for Calcium Imaging and All-Optical Physiology in Human Stem Cell-Derived Neurons.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- PinkyCaMP: an mScarlet-based calcium sensor with enhanced brightness, photostability and multiplexing capabilities.Nature methods · 2026Article
- Generalization of fear learning is shaped by inhibitory sensory processing in mice.Nature communications · 2026Article
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- Behavioral adaptation to warm conditions via Lim1-mediated acceleration of neuronal clocks.Nature neuroscience · 2026Article
- Social valence dictates sex differences in identity recognition.Translational psychiatry · 2026Article
- Calcium Imaging Studies in Enteric Glia.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Evaluation and experimental monitoring of calcium in plasma membrane inner nanoscale cytoplasm regions.Communications biology · 2025Article
- Calcium Imaging in Vivo: How to Correctly Select and Apply Fiber Optic Photometric Indicators.Organogenesis · 2025Review
- CYFIP1 governs the development of cortical axons by modulating calcium availability.Nature communications · 2025Article
670 more citing papers are in PubMed but not listed here.
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Authors and funding
32 authors.
Funding
Abstract
Genetically encoded calcium indicators (GECIs) are powerful tools for systems neuroscience. Recent efforts in protein engineering have significantly increased the performance of GECIs. The state-of-the art single-wavelength GECI, GCaMP3, has been deployed in a number of model organisms and can reliably detect three or more action potentials in short bursts in several systems in vivo. Through protein structure determination, targeted mutagenesis, high-throughput screening, and a battery of in vitro assays, we have increased the dynamic range of GCaMP3 by severalfold, creating a family of "GCaMP5" sensors. We tested GCaMP5s in several systems: cultured neurons and astrocytes, mouse retina, and in vivo in Caenorhabditis chemosensory neurons, Drosophila larval neuromuscular junction and adult antennal lobe, zebrafish retina and tectum, and mouse visual cortex. Signal-to-noise ratio was improved by at least 2- to 3-fold. In the visual cortex, two GCaMP5 variants detected twice as many visual stimulus-responsive cells as GCaMP3. By combining in vivo imaging with electrophysiology we show that GCaMP5 fluorescence provides a more reliable measure of neuronal activity than its predecessor GCaMP3. GCaMP5 allows more sensitive detection of neural activity in vivo and may find widespread applications for cellular imaging in general.
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