ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience2012
Analysis of synaptic growth and function in Drosophila with an extended larval stage.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Activity-Dependent Increases in Quantal Size at theJournal of developmental biology · 2025Article
- Immunohistochemical Analysis of the Drosophila Larval Neuromuscular Junction.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Drosophila postembryonic nervous system development: a model for the endocrine control of development.Genetics · 2023Review
- Regulation of presynaptic CaeLife · 2022Article
- Phosphatidylserine synthase plays an essential role in glia and affects development, as well as the maintenance of neuronal function.iScience · 2021Article
- Secreted C-type lectin regulation of neuromuscular junction synaptic vesicle dynamics modulates coordinated movement.Journal of cell science · 2021Article
- Synaptic homeostats: latent plasticity revealed at the Drosophila neuromuscular junction.Cellular and molecular life sciences : CMLS · 2021Review
- Developmental arrest ofDevelopment (Cambridge, England) · 2020Article
- Synaptic Properties and Plasticity Mechanisms of Invertebrate Tonic and Phasic Neurons.Frontiers in physiology · 2020Review
- Neurexin and Neuroligins Maintain the Balance of Ghost and Satellite Boutons at theFrontiers in neuroanatomy · 2020Article
- A Screen for Synaptic Growth Mutants Reveals Mechanisms That Stabilize Synaptic Strength.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Homeostatic scaling of active zone scaffolds maintains global synaptic strength.The Journal of cell biology · 2019Article
- Tao Negatively Regulates BMP Signaling During Neuromuscular Junction Development in Drosophila.Developmental neurobiology · 2019Article
- Two Drosophila model neurons can regenerate axons from the stump or from a converted dendrite, with feedback between the two sites.Neural development · 2017Article
- Secreted tissue inhibitor of matrix metalloproteinase restrictsJournal of cell science · 2017Article
- Article
- Coordinated movement, neuromuscular synaptogenesis and trans-synaptic signaling defects in Drosophila galactosemia models.Human molecular genetics · 2016Article
- Ligand-independent requirements of steroid receptors EcR and USP for cell survival.Cell death and differentiation · 2016Article
- Store-Operated Calcium Entry through Orai Is Required for Transcriptional Maturation of the Flight Circuit in Drosophila.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- Article
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3 authors.
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Abstract
The Drosophila larval neuromuscular junction (NMJ) is a powerful system for the genetic and molecular analysis of neuronal excitability, synaptic transmission, and synaptic development. However, its use for studying age-dependent processes, such as maintenance of neuronal viability and synaptic stability, are temporally limited by the onset of pupariation and metamorphosis. Here we characterize larval NMJ growth, growth regulation, structure, and function in a developmental variant with an extended third instar (ETI). RNAi-knockdown of the prothoracicotropic hormone receptor, torso, in the ring gland of developing larvae leaves the timing of first and second instar molts largely unchanged, but triples duration of the third instar from 3 to 9.5 d (McBrayer et al., 2007; Rewitz et al., 2009). During this ETI period, NMJs undergo additional growth (adding >50 boutons/NMJ), and this growth remains under the control of the canonical regulators Highwire and the TGFβ/BMP pathway. NMJ growth during the ETI period occurs via addition of new branches, satellite boutons, and interstitial boutons, and continues even after muscle growth levels off. Throughout the ETI, organization of synapses and active zones remains normal, and synaptic transmission is unchanged. These results establish the ETI larval system as a viable model for studying motor neuron diseases and for investigating time-dependent effects of perturbations that impair mechanisms of neuroprotection, synaptic maintenance, and response to neural injury.
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