Evidence map›Paper›PMID 23034122›Full record

ArticleGenome biology2012

Aging effects on DNA methylation modules in human brain and blood tissue.

Steve Horvath, Yafeng Zhang, Peter Langfelder, René S Kahn, Marco P M Boks, Kristel van Eijk, Leonard H van den Berg, Roel A Ophoff

Registry-linked trialOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genome biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03353597 (Reversing Epigenetic & Other Markers of Senescence by Transfusing Young Plasma To Older Human Subjects), which is not on this map. Cited by 398 papers, 8 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
398citing papers in PubMed, 8 pooled it
16.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT03353597 phase1 / phase2unknown statusnot on this mapstarted 2018, after this paper: background citation

Reversing Epigenetic & Other Markers of Senescence by Transfusing Young Plasma To Older Human Subjects

TypeinterventionalSponsorChandra DuggiralaRan2018 to 2022Enrolled2,120ConditionsAgingArmsPlasma Transfusion
3 · Its place in the literature

Who cites it

398 citing papers in PubMed, 8 syntheses or guidelines pooled it, 671 citations in OpenAlex.

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338 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Steve Horvath
Yafeng Zhang
Peter Langfelder
René S Kahn
Marco P M Boks
Kristel van Eijk
Leonard H van den Berg
Roel A Ophoff
University Medical Center Utrecht · NLUniversity of California, Los Angeles · US

Funding

UCLA SPORE IN PROSTATE CANCERP50CA092131 · NCI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI FREEMAN, MICHAEL R · 2002 to 2023
$42.6M
Genomic Studies of Bipolar Disorder in a Large Cohort from The NetherlandsR01MH090553 · NIMH · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI OPHOFF, ROEL A · 2010 to 2014
$6.0M
Epigenetics and disease: the role of DNA methylation in schizophrenia susceptibilR01DA028526 · NIDA · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI OPHOFF, ROEL A · 2009 to 2013
$2.5M
Genetics of Gene Expression and Gene Mapping for Amytrophic Lateral SclerosisR01NS058980 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI OPHOFF, ROEL A · 2008 to 2012
$1.6M
NCI NIH HHS P50 CA092131NCI NIH HHS P50CA092131NIDA NIH HHS R01 DA028526NIMH NIH HHS R01MH090553NINDS NIH HHS R01NS058980
6 · The paper itself

Abstract

backgroundSeveral recent studies reported aging effects on DNA methylation levels of individual CpG dinucleotides. But it is not yet known whether aging-related consensus modules, in the form of clusters of correlated CpG markers, can be found that are present in multiple human tissues. Such a module could facilitate the understanding of aging effects on multiple tissues.

resultsWe therefore employed weighted correlation network analysis of 2,442 Illumina DNA methylation arrays from brain and blood tissues, which enabled the identification of an age-related co-methylation module. Module preservation analysis confirmed that this module can also be found in diverse independent data sets. Biological evaluation showed that module membership is associated with Polycomb group target occupancy counts, CpG island status and autosomal chromosome location. Functional enrichment analysis revealed that the aging-related consensus module comprises genes that are involved in nervous system development, neuron differentiation and neurogenesis, and that it contains promoter CpGs of genes known to be down-regulated in early Alzheimer's disease. A comparison with a standard, non-module based meta-analysis revealed that selecting CpGs based on module membership leads to significantly increased gene ontology enrichment, thus demonstrating that studying aging effects via consensus network analysis enhances the biological insights gained.

conclusionsOverall, our analysis revealed a robustly defined age-related co-methylation module that is present in multiple human tissues, including blood and brain. We conclude that blood is a promising surrogate for brain tissue when studying the effects of age on DNA methylation profiles.

Indexed as

DNA MethylationAdolescentAdultAgedAged, 80 and overAgingBrainChildChild, PreschoolCpG IslandsDatabases, GeneticEpigenesis, GeneticEpigenomicsFemaleHumansInfant

Identifiers

PMID23034122
PMCPMC4053733
OpenAlexW1990958459

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.