ArticleJournal of applied oral science : revista FOB
Buccal cells DNA extraction to obtain high quality human genomic DNA suitable for polymorphism genotyping by PCR-RFLP and Real-Time PCR.
Article in Journal of applied oral science : revista FOB. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
70 citing papers in PubMed, 163 citations in OpenAlex.
- Association between dental age variation and EGF, EGFR, TGFB1, and TGFB2 genetic polymorphisms.Journal of orofacial orthopedics = Fortschritte der Kieferorthopadie : Organ/official journal Deutsche Gesellschaft fur Kieferorthopadie · 2026Article
- Investigation of Genetic Polymorphisms in Inducible Nitric Oxide Synthase and Suppressor of Cytokine Signaling Genes and Pain After Root Canal Treatment.Biochemical genetics · 2026Article
- Association between single-nucleotide polymorphisms in endochondral development-related genes and 3D phenotypic variation of the cranial base.Head & face medicine · 2026Article
- A Lab-on-a-Chip for the Extraction and Analysis of Single Molecules of DNA from Biological Media.Nanomaterials (Basel, Switzerland) · 2026Article
- Application properties and biosafety enhancement in saliva collection: optimization of a non-blowing medical device.BMC oral health · 2026Article
- Exploring Persistent Apical Periodontitis in Humans: Integrative Genetic, Histological and Microbiological Perspectives for Translational Research.International endodontic journal · 2026Article
- Polymorphisms in SOD2 and SOD3 Genes are Associated with Dental Caries in Primary Dentition of Brazilian Children.Biochemical genetics · 2026Article
- Impact of Cytokine Genes on TMD and QoL in Construction Workers.Brazilian dental journal · 2026Article
- Condylar Bone Quality in Growing Children Is Associated With Genetic Polymorphisms in Genes Involved in Calcium and Phosphate Maintenance.BioMed research international · 2026Article
- Bio-inspired neutrosophic-enzyme intelligence framework for pediatric dental disease detection using multi-modal clinical data.Scientific reports · 2025Article
- Association between single nucleotide polymorphisms in vitamin D related and parathyroid hormone genes with nonsyndromic oral cleft.Journal of orofacial orthopedics = Fortschritte der Kieferorthopadie : Organ/official journal Deutsche Gesellschaft fur Kieferorthopadie · 2025Article
- Prevalence of Glutathione-S-Transferase T and M Deletion Polymorphisms in Apical Periodontitis: a Two-Center Observational Study.Acta stomatologica Croatica · 2025Article
- Involvement of the gene encoding the collagen type II alpha 1 chain in mandibular mobility.BMC oral health · 2025Article
- Is there an association between superoxide dismutase gene polymorphisms, antioxidants in oxidative stress pathway, and oral health-related quality of life after root canal treatment?Acta odontologica Scandinavica · 2025Article
- Article
- Article
- Polymorphisms and dental age in non-syndromic cleft lip and palate: a cross-sectional study.BMC pediatrics · 2025Article
- Exploring polymorphisms in genes encoding growth factors associated with non-syndromic cleft lip with or without cleft palate and tooth agenesis.Journal of applied oral science : revista FOB · 2025Article
- Investigating the impact of polymorphisms in theFrontiers in oral health · 2025Article
- Temporomandibular joint disorders, happiness, and COMT, HTR2A and FKBP5 polymorphisms in adolescents: a cross-sectional study.Brazilian oral research · 2025Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThe aim of this study was to evaluate, by PCR-RFLP and real-time PCR, the yield and quality of genomic DNA collected from buccal cells by mouthwash after different storage times at room temperature. MATERIAL AND
methodsA group of volunteers was recruited to collect buccal cells using a mouthwash solution. The collected solution was divided into 3 tubes, one tube were used for immediate extraction and the remaining received ethanol and were kept at room temperature for 4 and 8 days followed by dna extraction. The concentration, purity and integrity of the dna were determined using spectrophotometry and electrophoresis. DNA quality differences among the three incubation times were also evaluated for genotyping EGF +61 a/g (rs 4444903) polymorphism by PCR-RFLP and for IRF6 polymorphism (rs 17015215) using real-time PCR.
resultsThere was no significant difference of dna yield (p=0.75) and purity (p=0.86) among the three different incubation times. DNA obtained from different incubation times presented high-molecular weight. The PCR-RFLP and real time pcr reactions were successfully performed for all DNA samples, even those extracted after 8 days of incubation. All samples genotyped by real-time pcr presented c allele for irf6 gene polymorphism (homozygous: cc; heterozygous: Ct) and the C allele was used as a reference for Ct values. The samples presented the same genotype for the different times in both techniques.
conclusionWe demonstrated that the method described herein is simple and low cost, and that DNA can be extracted and pcr amplified after storage in mouthwash solution at room temperature.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.