ArticleJournal of the American Chemical Society2012
Molecular insights into the biosynthesis of guadinomine: a type III secretion system inhibitor.
Article in Journal of the American Chemical Society, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Multi-Omic Assessment of Microbial Communities and Their Polyketide Biosynthetic Potential Across Abyssal Sediments.Environmental microbiology · 2026Article
- Discovery of a Two-Step Enzyme Cascade Converting Aspartate to Aminomalonate in Peptide Natural Product Biosynthesis.Journal of the American Chemical Society · 2025Article
- Modifications of Acyl Carrier Protein-Bound Glycosylated Polyketides in Pactamycin Biosynthesis.Chemistry (Weinheim an der Bergstrasse, Germany) · 2023Article
- Research Progress on Small Molecular Inhibitors of the Type 3 Secretion System.Molecules (Basel, Switzerland) · 2022Review
- Natural products as antivibrio agents: insight into the chemistry and biological activity.RSC advances · 2022Review
- AvmM catalyses macrocyclization through dehydration/Michael-type addition in alchivemycin A biosynthesis.Nature communications · 2022Article
- The Genomic and Transcriptomic Analyses ofJournal of fungi (Basel, Switzerland) · 2021Article
- Article
- Heterologous Expression of Cryptomaldamide in a Cyanobacterial Host.ACS synthetic biology · 2020Article
- Article
- Macrocyclic colibactin induces DNA double-strand breaks via copper-mediated oxidative cleavage.Nature chemistry · 2019Article
- Natural Product Type III Secretion System Inhibitors.Antibiotics (Basel, Switzerland) · 2019Review
- Extender Unit Promiscuity and Orthogonal Protein Interactions of an Aminomalonyl-ACP Utilizing Trans-Acyltransferase from Zwittermicin Biosynthesis.ACS chemical biology · 2018Article
- Total Synthesis of (+)-Guadinomic Acid via Hydroxyl-Directed Guanidylation.The Journal of organic chemistry · 2018Article
- Analysis of the Pseudouridimycin Biosynthetic Pathway Provides Insights into the Formation of C-nucleoside Antibiotics.Cell chemical biology · 2018Article
- Direct Amination of α-Hydroxy Amides.Asian journal of organic chemistry · 2017Article
- The Uncommon Enzymology of Cis-Acyltransferase Assembly Lines.Chemical reviews · 2017Review
- Linking Biosynthetic Gene Clusters to their Metabolites via Pathway- Targeted Molecular Networking.Current topics in medicinal chemistry · 2016Review
- The re-emergence of natural products for drug discovery in the genomics era.Nature reviews. Drug discovery · 2015Review
- Computational identification and analysis of orphan assembly-line polyketide synthases.The Journal of antibiotics · 2014Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Guadinomines are a recently discovered family of anti-infective compounds produced by Streptomyces sp. K01-0509 with a novel mode of action. With an IC(50) of 14 nM, guadinomine B is the most potent known inhibitor of the type III secretion system (TTSS) of Gram-negative bacteria. TTSS activity is required for the virulence of many pathogenic Gram-negative bacteria including Escherichia coli , Salmonella spp., Yersinia spp., Chlamydia spp., Vibrio spp., and Pseudomonas spp. The guadinomine (gdn) biosynthetic gene cluster has been cloned and sequenced and includes 26 open reading frames spanning 51.2 kb. It encodes a chimeric multimodular polyketide synthase, a nonribosomal peptide synthetase, along with enzymes responsible for the biosynthesis of the unusual aminomalonyl-acyl carrier protein extender unit and the signature carbamoylated cyclic guanidine. Its identity was established by targeted disruption of the gene cluster as well as by heterologous expression and analysis of key enzymes in the biosynthetic pathway. Identifying the guadinomine gene cluster provides critical insight into the biosynthesis of these scarce but potentially important natural products.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.