ArticlePloS one2012
Human amnion epithelial cells induced to express functional cystic fibrosis transmembrane conductance regulator.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.
- Application of human amniotic epithelial cells in regenerative medicine: a systematic review.Stem cell research & therapy · 2020Pooled it
- Efficient Gene Editing at Major CFTR Mutation Loci.Molecular therapy. Nucleic acids · 2019Article
- A tunable hydrogel system for long-term release of cell-secreted cytokines and bioprinted in situ wound cell delivery.Journal of biomedical materials research. Part B, Applied biomaterials · 2017Article
- Concise Review: Fetal Membranes in Regenerative Medicine: New Tricks from an Old Dog?Stem cells translational medicine · 2017Review
- Amnion Epithelial Cells Promote Lung Repair via Lipoxin AStem cells translational medicine · 2017Article
- Human amnion epithelial cells modulate the inflammatory response to ventilation in preterm lambs.PloS one · 2017Article
- Use of trimetasphere metallofullerene MRI contrast agent for the non-invasive longitudinal tracking of stem cells in the lung.Methods (San Diego, Calif.) · 2016Article
- Endogenous and Exogenous Stem/Progenitor Cells in the Lung and Their Role in the Pathogenesis and Treatment of Pediatric Lung Disease.Frontiers in pediatrics · 2016Review
- Three-dimensional imaging of human stem cells using soft X-ray tomography.Journal of the Royal Society, Interface · 2015Article
- Isolation, cryopreservation and culture of human amnion epithelial cells for clinical applications.Journal of visualized experiments : JoVE · 2014Article
- Maintenance of human amnion epithelial cell phenotype in pulmonary surfactant.Stem cell research & therapy · 2014Article
- Correction of defective CFTR/ENaC function and tightness of cystic fibrosis airway epithelium by amniotic mesenchymal stromal (stem) cells.Journal of cellular and molecular medicine · 2014Article
- Stem cells, cell therapies, and bioengineering in lung biology and diseases. Comprehensive review of the recent literature 2010-2012.Annals of the American Thoracic Society · 2013Review
- Gram-negative bacterial lipopolysaccharide stimulates activin a secretion from human amniotic epithelial cells.International journal of endocrinology · 2013Article
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 2 countries.
Funding
Abstract
Cystic fibrosis, an autosomal recessive disorder caused by a mutation in a gene encoding the cystic fibrosis transmembrane conductance regulator (CFTR), remains a leading cause of childhood respiratory morbidity and mortality. The respiratory consequences of cystic fibrosis include the generation of thick, tenacious mucus that impairs lung clearance, predisposing the individual to repeated and persistent infections, progressive lung damage and shortened lifespan. Currently there is no cure for cystic fibrosis. With this in mind, we investigated the ability of human amnion epithelial cells (hAECs) to express functional CFTR. We found that hAECs formed 3-dimensional structures and expressed the CFTR gene and protein after culture in Small Airway Growth Medium (SAGM). We also observed a polarized CFTR distribution on the membrane of hAECs cultured in SAGM, similar to that observed in polarized airway cells in vivo. Further, hAECs induced to express CFTR possessed functional iodide/chloride (I(-/)Cl(-)) ion channels that were inhibited by the CFTR-inhibitor CFTR-172, indicating the presence of functional CFTR ion channels. These data suggest that hAECs may be a promising source for the development of a cellular therapy for cystic fibrosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.