ArticlePloS one2012
Enhanced adhesion of Campylobacter jejuni to abiotic surfaces is mediated by membrane proteins in oxygen-enriched conditions.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 56 citations in OpenAlex.
- Pathogenicity and virulence ofVirulence · 2024Review
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- Evaluation of physical and chemical isolation methods to extract and purifyFrontiers in microbiology · 2024Article
- Relevance and Importance of Biofilms in the Resistance and Spreading of Campylobacter spp. Within the Food Chain.Advances in experimental medicine and biology · 2023Article
- Comparative Analysis of L-Fucose Utilization and Its Impact on Growth and Survival ofFrontiers in microbiology · 2022Article
- Metaphenotypes associated with recurrent genomic lineages ofFrontiers in microbiology · 2022Article
- A novel high-content screening approach for the elucidation of C. jejuni biofilm composition and integrity.BMC microbiology · 2021Article
- Multi-Omics Approach Reveals the Potential Core Vaccine Targets for the Emerging Foodborne PathogenFrontiers in microbiology · 2021Article
- Antibiotic Resistance in the Alternative Lifestyles ofFrontiers in cellular and infection microbiology · 2021Article
- Bridging the Gap: A Role forMicroorganisms · 2020Review
- Adhesion ofMicroorganisms · 2020Article
- Membrane Proteocomplexome ofFrontiers in microbiology · 2020Article
- Adhesion, Biofilm Formation, andFrontiers in cellular and infection microbiology · 2020Article
- Article
- Peptide Ligands of AmiA, AliA, and AliB Proteins Determine Pneumococcal Phenotype.Frontiers in microbiology · 2018Article
- Enhanced Biofilm Formation by Ferrous and Ferric Iron Through Oxidative Stress inFrontiers in microbiology · 2018Article
- Transcriptome and proteome dynamics in chemostat culture reveal how Campylobacter jejuni modulates metabolism, stress responses and virulence factors upon changes in oxygen availability.Environmental microbiology · 2017Article
- Intrinsic and Extrinsic Aspects onFrontiers in microbiology · 2017Article
- Comparative Proteomics Reveals Differences in Host-Pathogen Interaction between Infectious and Commensal Relationship withFrontiers in cellular and infection microbiology · 2017Article
- Draft Genome Sequences of Three Multiantibiotic-Resistant Campylobacter jejuni Strains (2865, 2868, and 2871) Isolated from Poultry at Retail Outlets in Malaysia.Genome announcements · 2016Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Campylobacter jejuni is responsible for the major foodborne bacterial enteritis in humans. In contradiction with its fastidious growth requirements, this microaerobic pathogen can survive in aerobic food environments, suggesting that it must employ a variety of protection mechanisms to resist oxidative stress. For the first time, C. jejuni 81-176 inner and outer membrane subproteomes were analyzed separately using two-dimensional protein electrophoresis (2-DE) of oxygen-acclimated cells and microaerobically grown cells. LC-MS/MS analyses successfully identified 42 and 25 spots which exhibited a significantly altered abundance in the IMP-enriched fraction and in the OMP-enriched fraction, respectively, in response to oxidative conditions. These spots corresponded to 38 membrane proteins that could be grouped into different functional classes: (i) transporters, (ii) chaperones, (iii) fatty acid metabolism, (iv) adhesion/virulence and (v) other metabolisms. Some of these proteins were up-regulated at the transcriptional level in oxygen-acclimated cells as confirmed by qRT-PCR. Downstream analyses revealed that adhesion of C. jejuni to inert surfaces and swarming motility were enhanced in oxygen-acclimated cells or paraquat-stressed cells, which could be explained by the higher abundance of membrane proteins involved in adhesion and biofilm formation. The virulence factor CadF, over-expressed in the outer membrane of oxygen-acclimated cells, contributes to the complex process of C. jejuni adhesion to inert surfaces as revealed by a reduction in the capability of C. jejuni 81-176 ΔCadF cells compared to the isogenic strain.Taken together, these data demonstrate that oxygen-enriched conditions promote the over-expression of membrane proteins involved in both the biofilm initiation and virulence of C. jejuni.
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Registered trials
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