ArticlePloS one2012
Partial and full PCR-based reverse genetics strategy for influenza viruses.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- Viral expansion after transfer is a primary driver of influenza A virus transmission bottlenecks.PLoS biology · 2025Article
- Viral expansion after transfer is a primary driver of influenza A virus transmission bottlenecks.bioRxiv : the preprint server for biology · 2025Article
- Developments in Negative-Strand RNA Virus Reverse Genetics.Microorganisms · 2024Review
- Intra- and inter-host evolution of H9N2 influenza A virus in Japanese quail.Virus evolution · 2022Article
- Evolution and Antigenic Advancement of N2 Neuraminidase of Swine Influenza A Viruses Circulating in the United States following Two Separate Introductions from Human Seasonal Viruses.Journal of virology · 2021Article
- Influenza Reverse Genetics-Historical Perspective.Cold Spring Harbor perspectives in medicine · 2021Review
- Development of a swine RNA polymerase I driven Influenza reverse genetics system for the rescue of type A and B Influenza viruses.Journal of virological methods · 2021Article
- Collective interactions augment influenza A virus replication in a host-dependent manner.Nature microbiology · 2020Article
- PCR-based reverse genetics strategy for bluetongue virus recovery.Virology journal · 2019Article
- Incomplete influenza A virus genomes occur frequently but are readily complemented during localized viral spread.Nature communications · 2019Article
- FlexibilityJournal of virology · 2019Article
- Plasticity of Amino Acid Residue 145 Near the Receptor Binding Site of H3 Swine Influenza A Viruses and Its Impact on Receptor Binding and Antibody Recognition.Journal of virology · 2019Article
- Alternative reverse genetics system for influenza viruses based on a synthesized swine 45S rRNA promoter.Virus genes · 2017Article
- Heterologous Packaging Signals on Segment 4, but Not Segment 6 or Segment 8, Limit Influenza A Virus Reassortment.Journal of virology · 2017Article
- The Molecular Determinants of Antibody Recognition and Antigenic Drift in the H3 Hemagglutinin of Swine Influenza A Virus.Journal of virology · 2016Article
- Identification of Influenza A/PR/8/34 Donor Viruses Imparting High Hemagglutinin Yields to Candidate Vaccine Viruses in Eggs.PloS one · 2015Article
- All-in-one bacmids: an efficient reverse genetics strategy for influenza A virus vaccines.Journal of virology · 2014Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Since 1999, plasmid-based reverse genetics (RG) systems have revolutionized the way influenza viruses are studied. However, it is not unusual to encounter cloning difficulties for one or more influenza genes while attempting to recover virus de novo. To overcome some of these shortcomings we sought to develop partial or full plasmid-free RG systems. The influenza gene of choice is assembled into a RG competent unit by virtue of overlapping PCR reactions containing a cDNA copy of the viral gene segment under the control of RNA polymerase I promoter (pol1) and termination (t1) signals - herein referred to as Flu PCR amplicons. Transfection of tissue culture cells with either HA or NA Flu PCR amplicons and 7 plasmids encoding the remaining influenza RG units, resulted in efficient virus rescue. Likewise, transfections including both HA and NA Flu PCR amplicons and 6 RG plasmids also resulted in efficient virus rescue. In addition, influenza viruses were recovered from a full set of Flu PCR amplicons without the use of plasmids.
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