ArticlePloS one2012
Induction of the staphylococcal proteolytic cascade by antimicrobial fatty acids in community acquired methicillin resistant Staphylococcus aureus.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 53 citations in OpenAlex.
- Differential pathogenic and commensal response ofJournal of bacteriology · 2026Article
- An anti-adhesive compound modulating the production of Staphylococcus aureus cell wall-anchored proteins.Nature communications · 2026Article
- Lung surfactant reducesMicrobiology spectrum · 2025Article
- Activity-Based Protein Profiling Identifies an α-Amylase Family Protein Contributing to the Virulence of Methicillin-ResistantACS infectious diseases · 2025Article
- Prophages divert Staphylococcus aureus defenses against host lipids.Journal of lipid research · 2024Article
- The Phospholipase A1 Activity of Glycerol Ester Hydrolase (Geh) Is Responsible for Extracellular 2-12(mSphere · 2023Article
- Repeated Emergence of Variant TetR Family Regulator, FarR, and Increased Resistance to Antimicrobial Unsaturated Fatty Acid among Clonal Complex 5 Methicillin-Resistant Staphylococcus aureus.Antimicrobial agents and chemotherapy · 2023Article
- Sacha Inchi (Heliyon · 2022Review
- Article
- Prevalence and Characterization of Coagulase PositiveAntibiotics (Basel, Switzerland) · 2021Article
- Prevalence and Characteristics ofFrontiers in microbiology · 2021Article
- Unsaturated Fatty Acids Control Biofilm Formation ofAntibiotics (Basel, Switzerland) · 2020Article
- Novel Functions and Signaling Specificity for the GraS Sensor Kinase of Staphylococcus aureus in Response to Acidic pH.Journal of bacteriology · 2020Article
- Staphylococcus aureus Releases Proinflammatory Membrane Vesicles To Resist Antimicrobial Fatty Acids.mSphere · 2020Article
- The type VII secretion system protects Staphylococcus aureus against antimicrobial host fatty acids.Scientific reports · 2020Article
- DNA Binding and Sensor Specificity of FarR, a Novel TetR Family Regulator Required for Induction of the Fatty Acid Efflux Pump FarE inJournal of bacteriology · 2019Article
- SbnI is a free serine kinase that generatesThe Journal of biological chemistry · 2018Article
- Untargeted lipidomic analysis to broadly characterize the effects of pathogenic and non-pathogenic staphylococci on mammalian lipids.PloS one · 2018Article
- The role of two branched-chain amino acid transporters in Staphylococcus aureus growth, membrane fatty acid composition and virulence.Molecular microbiology · 2016Article
- Lipoproteins of Gram-Positive Bacteria: Key Players in the Immune Response and Virulence.Microbiology and molecular biology reviews : MMBR · 2016Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Community acquired methicillin resistant Staphylococcus aureus (CA-MRSA), and the USA300 strain of CA-MRSA in particular, are known for their rapid community transmission, and propensity to cause aggressive skin and soft tissue infections. To assess factors that contribute to these hallmark traits of CA-MRSA, we evaluated how growth of USA300 and production of secreted virulence factors was influenced on exposure to physiologic levels of unsaturated free fatty acids that would be encountered on the skin or anterior nares, which represent the first sites of contact with healthy human hosts. There was a sharp threshold between sub-inhibitory and inhibitory concentrations, such that 100 µM sapienic acid (C16∶1) and linoleic acid (C18∶1) were sufficient to prevent growth after 24 h incubation, while 25 µM allowed unrestricted growth, and 50 µM caused an approximate 10-12 h lag, followed by unimpeded exponential growth. Conversely, saturated palmitic or stearic acids did not affect growth at 100 µM. Although growth was not affected by 25 µM sapienic or linoleic acid, these and other unsaturated C16 and C18 fatty acids, but not their saturated counterparts, promoted robust production of secreted proteases comprising the Staphylococcal proteolytic cascade. This trait was also manifested to varying degrees in other CA-MRSA, and in genetically diverse methicillin susceptible S. aureus strains. Therefore, induction of the Staphylococcal proteolytic cascade by unsaturated fatty acids is another feature that should now be evaluated as a potential contributing factor in the aggressive nature of skin and soft tissue infections caused by USA300, and as a general virulence mechanism of S. aureus.
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Registered trials
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