ArticlePloS one2012
Implication of glutathione in the in vitro antiplasmodial mechanism of action of ellagic acid.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 32 citations in OpenAlex.
- Glutathione redox system as a therapeutic target for Plasmodium: Opportunities, Challenges, and future perspectives.Folia microbiologica · 2026Review
- Eschweilenol C and subfractions from Terminalia plants disrupt haemoglobin metabolism, inhibiting Plasmodium falciparum growth at rings and trophozoite stages.Malaria journal · 2025Article
- Article
- Ellagic acid and allopurinol decrease HExperimental brain research · 2022Article
- Suppressive, Curative, and Prophylactic Effects ofBioMed research international · 2022Article
- Article
- Identification of 3,3'-O-dimethylellagic acid and apigenin as the main antiplasmodial constituents of Endodesmia calophylloides Benth and Hymenostegia afzelii (Oliver.) Harms.BMC complementary medicine and therapies · 2021Article
- In Vitro Antimalarial and Toxicological Activities ofThe Malaysian journal of medical sciences : MJMS · 2020Article
- Ellagic acid microspheres restrict the growth of Babesia and Theileria in vitro and Babesia microti in vivo.Parasites & vectors · 2019Article
- Glucose 6-phosphate dehydrogenase 6-phosphogluconolactonase: characterization of the Plasmodium vivax enzyme and inhibitor studies.Malaria journal · 2019Article
- Antimalarial activity of hydromethanolic extract and its solvent fractions ofSAGE open medicine · 2019Article
- Liver-Stage Specific Response among Endemic Populations: Diet and Immunity.Frontiers in immunology · 2015Review
- Antimalarial action of artesunate involves DNA damage mediated by reactive oxygen species.Antimicrobial agents and chemotherapy · 2015Article
- The role of antioxidants treatment on the pathogenesis of malarial infections: a review.Parasitology research · 2014Review
- In vitro antiplasmodial activity of some medicinal plants of Burkina Faso.Parasitology research · 2014Article
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The search for new antimalarial chemotherapy has become increasingly urgent due to parasite resistance to current drugs. Ellagic acid (EA) is a polyphenol, recently found in various plant products, that has effective antimalarial activity in vitro and in vivo without toxicity. To further understand the antimalarial mechanism of action of EA in vitro, we evaluated the effects of EA, ascorbic acid and N-acetyl-L-cysteine (NAC), alone and/or in combination on the production of reactive oxygen species (ROS) during the trophozoite and schizonte stages of the erythrocytic cycle of P. falciparum. The parasitized erythrocytes were pre-labelled with DCFDA (dichlorofluorescein diacetate). We showed that NAC had no effect on ROS production, contrary to ascorbic acid and EA, which considerably reduced ROS production. Surprisingly, EA reduced the production of the ROS with concentrations (6.6×10(-9) - 6.6×10(-6) M) ten-fold lower than ascorbic acid (113×10(-6) M). Additionally, the in vitro drug sensitivity of EA with antioxidants showed that antiplasmodial activity is independent of the ROS production inside parasites, which was confirmed by the additive activity of EA and desferrioxamine. Finally, EA could act by reducing the glutathione content inside the Plasmodium parasite. This was consolidated by the decrease in the antiplasmodial efficacy of EA in the murine model Plasmodium yoelii- high GSH strain, known for its high glutathione content. Given its low toxicity and now known mechanism of action, EA appears as a promising antiplasmodial compound.
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