ArticlePloS one2012
Degradation of PsbO by the Deg protease HhoA Is thioredoxin dependent.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Spatial proteomics reveals signal sequence characteristics correlated with localization in cyanobacteria.Plant physiology · 2025Article
- Structural basis for an early stage of the photosystem II repair cycle in Chlamydomonas reinhardtii.Nature communications · 2024Article
- The lifetime of the oxygen-evolving complex subunit PSBO depends on light intensity and carbon availability in Chlamydomonas.Plant, cell & environment · 2023Article
- Article
- Proximity Labeling Facilitates Defining the Proteome Neighborhood of Photosystem II Oxygen Evolution Complex in a Model Cyanobacterium.Molecular & cellular proteomics : MCP · 2022Article
- Current Knowledge on Mechanisms Preventing Photosynthesis Redox Imbalance in Plants.Antioxidants (Basel, Switzerland) · 2021Review
- Proximity-based proteomics reveals the thylakoid lumen proteome in the cyanobacterium Synechococcus sp. PCC 7002.Photosynthesis research · 2021Article
- The evolutionarily conserved HtrA is associated with stress tolerance and protein homeostasis in the halotolerant cyanobacterium Halothece sp. PCC7418.Extremophiles : life under extreme conditions · 2020Article
- Molecular Basis Underlying Leaf Variegation of a Moth Orchid Mutant (Frontiers in plant science · 2017Article
- The Role of Light-Dark Regulation of the Chloroplast ATP Synthase.Frontiers in plant science · 2017Article
- Insights into the Cyanobacterial Deg/HtrA Proteases.Frontiers in plant science · 2016Article
- The conservative cysteines in transmembrane domain of AtVKOR/LTO1 are critical for photosynthetic growth and photosystem II activity in Arabidopsis.Frontiers in plant science · 2015Article
- Conditional, temperature-induced proteolytic regulation of cyanobacterial RNA helicase expression.Journal of bacteriology · 2014Article
- Proteomic evidences for rex regulation of metabolism in toxin-producing Bacillus cereus ATCC 14579.PloS one · 2014Article
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Authors and funding
5 authors.
Funding
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Abstract
The widely distributed members of the Deg/HtrA protease family play an important role in the proteolysis of misfolded and damaged proteins. Here we show that the Deg protease rHhoA is able to degrade PsbO, the extrinsic protein of the Photosystem II (PSII) oxygen-evolving complex in Synechocystis sp. PCC 6803 and in spinach. PsbO is known to be stable in its oxidized form, but after reduction by thioredoxin it became a substrate for recombinant HhoA (rHhoA). rHhoA cleaved reduced eukaryotic (specifically, spinach) PsbO at defined sites and created distinct PsbO fragments that were not further degraded. As for the corresponding prokaryotic substrate (reduced PsbO of Synechocystis sp. PCC 6803), no PsbO fragments were observed. Assembly to PSII protected PsbO from degradation. For Synechocystis sp. PCC 6803, our results show that HhoA, HhoB, and HtrA are localized in the periplasma and/or at the thylakoid membrane. In agreement with the idea that PsbO could be a physiological substrate for Deg proteases, part of the cellular fraction of the three Deg proteases of Synechocystis sp. PCC 6803 (HhoA, HhoB, and HtrA) was detected in the PSII-enriched membrane fraction.
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