ArticlePloS one2012
Distance and character-based evaluation of the V4 region of the 18S rRNA gene for the identification of diatoms (Bacillariophyceae).
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 72 citations in OpenAlex.
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- Local diversity of heathland Cercozoa explored by in-depth sequencing.The ISME journal · 2016Article
- R-Syst::diatom: an open-access and curated barcode database for diatoms and freshwater monitoring.Database : the journal of biological databases and curation · 2016Article
- Biogeography of heterotrophic flagellate populations indicates the presence of generalist and specialist taxa in the Arctic Ocean.Applied and environmental microbiology · 2015Article
- High-Throughput Sequencing-The Key to Rapid Biodiversity Assessment of Marine Metazoa?PloS one · 2015Article
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- Pyrosequencing analysis of the protist communities in a High Arctic meromictic lake: DNA preservation and change.Frontiers in microbiology · 2012Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA barcoding is a molecular tool that exploits a unique DNA sequence of a standardized gene or non-coding region for the species identification of unknown individuals. The investigation into a suitable barcode for diatoms is ongoing and there are several promising candidates including mitochondrial, plastidial and nuclear markers. We analyzed 272 sequences from 76 diatoms species in the orders Thalassiosirales, Lithodesmiales and Cymatosirales, using distance and character based approaches, to assess the applicability of a DNA barcode based on the hypervariable V4 region of the nuclear 18S rRNA gene. We show that the proposed V4 barcode separated ca. 97% of all centric diatom taxa tested using a threshold p-distance of 0.02 and that many problem pairs were further separated using a character based approach. The reliability of amplification, extensive reference library and variability seen in the V4 region make it the most promising candidate to date for a barcode marker for diatoms particularly when combined with DNA character analysis.
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