ArticlePloS one2012
A lateral flow assay for quantitative detection of amplified HIV-1 RNA.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 72 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
72 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Evaluation of Lateral-Flow Assay for Rapid Detection of Influenza Virus.BioMed research international · 2020Pooled it
- Yes/No Quantitative Analysis of Single-Stranded Oligonucleotides with Lateral Flow Assays.Biosensors · 2026Article
- Article
- Electrochemical Lateral Flow Platforms: Pioneering the Future of Rapid Testing.Molecules (Basel, Switzerland) · 2026Review
- Review
- Development of a Protein-Free Nucleic Acid Lateral Flow Assay forAnalytical chemistry · 2025Article
- Design of a lateral flow assay targeting the conserved NIID_2019-nCoV_N gene region for molecular viral diagnosis.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2025Article
- Overcoming Limited Access to Virus Infection Rapid Testing: Development of a Lateral Flow Test for SARS-CoV-2 with Locally Available Resources.Biosensors · 2024Article
- Development and evaluation of a high-sensitivity RT-PCR lateral flow assay for early detection of HIV-1 infection.Heliyon · 2024Article
- Direct Detection of Viral Infections from Swab Samples by Probe-Gated Silica Nanoparticle-Based Lateral Flow Assay.ChemistryOpen · 2024Article
- Lateral Flow Assay for Hepatitis B Detection: A Review of Current and New Assays.Micromachines · 2023Review
- CRISPR‑based diagnostic approaches: Implications for rapid management of future pandemics (Review).Molecular medicine reports · 2023Review
- Development of a rapid and ultra-sensitive RNA:DNA hybrid immunocapture based biosensor for visual detection of SARS-CoV-2 RNA.PNAS nexus · 2023Article
- Development of A Rapid, Low-Cost Portable Detection Assay for Enterococci in Wastewater and Environmental Waters.Microorganisms · 2023Article
- Lateral flow assays for viruses diagnosis: Up-to-date technology and future prospects.Trends in analytical chemistry : TRAC · 2022Review
- Development of a Multiplex Polymerase Chain Reaction-Based DNA Lateral Flow Assay as a Point-of-Care Diagnostic for Fast and Simultaneous Detection of MRSA and Vancomycin Resistance in Bacteremia.Diagnostics (Basel, Switzerland) · 2022Article
- Lateral Flow Immunoassays for Detecting Viral Infectious Antigens and Antibodies.Micromachines · 2022Review
- Onsite detection of plant viruses using isothermal amplification assays.Plant biotechnology journal · 2022Review
- Near-digital amplification in paper improves sensitivity and speed in biplexed reactions.Scientific reports · 2022Article
- Characterization of wax valving and μPIV analysis of microscale flow in paper-fluidic devices for improved modeling and design.Lab on a chip · 2022Article
12 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Although the accessibility of HIV treatment in developing nations has increased dramatically over the past decade, viral load testing to monitor the response of patients receiving therapy is often unavailable. Existing viral load technologies are often too expensive or resource-intensive for poor settings, and there is no appropriate HIV viral load test currently available at the point-of-care in low resource settings. Here, we present a lateral flow assay that employs gold nanoparticle probes and gold enhancement solution to detect amplified HIV RNA quantitatively. Preliminary results show that, when coupled with nucleic acid sequence based amplification (NASBA), this assay can detect concentrations of HIV RNA that match the clinically relevant range of viral loads found in HIV patients. The lateral flow test is inexpensive, simple and rapid to perform, and requires few resources. Our results suggest that the lateral flow assay may be integrated with amplification and sample preparation technologies to serve as an HIV viral load test for low-resource settings.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.