ArticlePloS one2012
Molecular characterization of the viaB locus encoding the biosynthetic machinery for Vi capsule formation in Salmonella Typhi.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 42 citations in OpenAlex.
- The Manipulation of Host Cells byInternational journal of molecular sciences · 2026Review
- Protein-centric mechanisms of typhoidalFrontiers in cellular and infection microbiology · 2026Review
- Genomic and antimicrobial susceptibility characterization of a clinicalFrontiers in cellular and infection microbiology · 2026Article
- Genetic engineering of E. coli K-12 for heterologous carbohydrate antigen production.Microbial cell factories · 2025Article
- Assessing Salmonella Typhi Pathogenicity and Prevention: The Crucial Role of Vaccination in Combating Typhoid Fever.International journal of molecular sciences · 2025Review
- Genetic heterogeneity in theMicrobial genomics · 2024Article
- Single missense mutations in Vi capsule synthesis genes confer hypervirulence to Salmonella Typhi.Nature communications · 2024Article
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- Expression of a Shiga-Like Toxin during Plastic Colonization by Two Multidrug-Resistant Bacteria,Microorganisms · 2020Article
- Genomic analysis of Indian strains of Salmonella enterica subsp. enterica serovar Typhi indicates novel genetic repertoire for pathogenicity and adaptations.Molecular biology reports · 2019Article
- Adsorption of Vi Capsular Antigen ofPolymers · 2019Article
- Periplasmic depolymerase provides insight into ABC transporter-dependent secretion of bacterial capsular polysaccharides.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Potential targets for next generation antimicrobial glycoconjugate vaccines.FEMS microbiology reviews · 2018Review
- Mechanisms to Evade the Phagocyte Respiratory Burst Arose by Convergent Evolution in Typhoidal Salmonella Serovars.Cell reports · 2018Article
- Bi-valent polysaccharides of Vi capsular and O9 O-antigen in attenuatedNPJ vaccines · 2018Article
- Unique lipid anchor attaches Vi antigen capsule to the surface of Salmonella enterica serovar Typhi.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Global MLST of Salmonella Typhi Revisited in Post-genomic Era: Genetic Conservation, Population Structure, and Comparative Genomics of Rare Sequence Types.Frontiers in microbiology · 2016Article
- Now you see me, now you don't: the interaction of Salmonella with innate immune receptors.Nature reviews. Microbiology · 2015Review
- Simplest identification, O-specific polysaccharide purification and antigenic evaluation of Salmonella enterica serovar Typhi Vi negative isolate.EXCLI journal · 2015Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Vi capsular polysaccharide (CPS) of Salmonella enterica serovar Typhi, the cause of human typhoid, is important for infectivity and virulence. The Vi biosynthetic machinery is encoded within the viaB locus composed of 10 genes involved in regulation of expression (tviA), polymer synthesis (tviB-tviE), and cell surface localization of the CPS (vexA-vexE). We cloned the viaB locus from S. Typhi and transposon insertion mutants of individual viaB genes were characterized in Escherichia coli DH5α. Phenotype analysis of viaB mutants revealed that tviB, tviC, tviD and tviE are involved in Vi polymer synthesis. Furthermore, expression of tviB-tviE in E. coli DH5α directed the synthesis of cytoplasmic Vi antigen. Mutants of the ABC transporter genes vexBC and the polysaccharide copolymerase gene vexD accumulated the Vi polymer within the cytoplasm and productivity in these mutants was greatly reduced. In contrast, de novo synthesis of Vi polymer in the export deficient vexA mutant was comparable to wild-type cells, with drastic effects on cell stability. VexE mutant cells exported the Vi, but the CPS was not retained at the cell surface. The secreted polymer of a vexE mutant had different physical characteristics compared to the wild-type Vi.
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