ArticlePloS one2012
Lactobacillus rhamnosus accelerates zebrafish backbone calcification and gonadal differentiation through effects on the GnRH and IGF systems.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 138 citations in OpenAlex.
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- Shifting seas and first feeds: gut microbiome dynamics in juvenile chum salmon (Current research in microbial sciences · 2025Article
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- Causal relationship between gut microbiota and insulin-like growth factor 1: a bidirectional two-sample Mendelian randomization study.Frontiers in cellular and infection microbiology · 2024Article
- Sustainable Ornamental Fish Aquaculture: The Implication of Microbial Feed Additives.Animals : an open access journal from MDPI · 2023Review
- The associations of gut microbiota, endocrine system and bone metabolism.Frontiers in microbiology · 2023Review
- Turning the tide on sex and the microbiota in aquatic animals.Hydrobiologia · 2023Article
- Zebrafish: an efficient vertebrate model for understanding role of gut microbiota.Molecular medicine (Cambridge, Mass.) · 2022Review
- Application of zebrafish in the study of the gut microbiome.Animal models and experimental medicine · 2022Review
- Soybean Meal-Dependent Acute Intestinal Inflammation Delays Osteogenesis in Zebrafish Larvae.International journal of molecular sciences · 2022Article
- Zebrafish caudal fin as a model to investigate the role of probiotics in bone regeneration.Scientific reports · 2022Article
- The Mediating Role of the Gut Microbiota in the Physical Growth of Children.Life (Basel, Switzerland) · 2022Review
- Characteristics of Gut Microbiome and Its Metabolites, Short-Chain Fatty Acids, in Children With Idiopathic Short Stature.Frontiers in endocrinology · 2022Article
- Tenets in Microbial Endocrinology: A New Vista in Teleost Reproduction.Frontiers in physiology · 2022Review
Corrections and comments
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Authors and funding
7 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Endogenous microbiota play essential roles in the host's immune system, physiology, reproduction and nutrient metabolism. We hypothesized that a continuous administration of an exogenous probiotic might also influence the host's development. Thus, we treated zebrafish from birth to sexual maturation (2-months treatment) with Lactobacillus rhamnosus, a probiotic species intended for human use. We monitored for the presence of L. rhamnosus during the entire treatment. Zebrafish at 6 days post fertilization (dpf) exhibited elevated gene expression levels for Insulin-like growth factors -I and -II, Peroxisome proliferator activated receptors -α and -β, VDR-α and RAR-γ when compared to untreated-10 days old zebrafish. Using a gonadotropin-releasing hormone 3 GFP transgenic zebrafish (GnRH3-GFP), higher GnRH3 expression was found at 6, 8 and 10 dpf upon L. rhamnosus treatment. The same larvae exhibited earlier backbone calcification and gonad maturation. Noteworthy in the gonad development was the presence of first testes differentiation at 3 weeks post fertilization in the treated zebrafish population -which normally occurs at 8 weeks- and a dramatic sex ratio modulation (93% females, 7% males in control vs. 55% females, 45% males in the treated group). We infer that administration of L. rhamnosus stimulated the IGF system, leading to a faster backbone calcification. Moreover we hypothesize a role for administration of L. rhamnosus on GnRH3 modulation during early larval development, which in turn affects gonadal development and sex differentiation. These findings suggest a significant role of the microbiota composition on the host organism development profile and open new perspectives in the study of probiotics usage and application.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.