ArticlePloS one2012
Genomic integration and germline transmission of plasmid injected into crustacean Daphnia magna eggs.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 53 citations in OpenAlex.
- HP1-Mediated Silencing of theJournal of developmental biology · 2025Article
- Baculovirus and Plasmid Vector-Mediated Gene Transfer inBio-protocol · 2025Article
- The mosaicism of Cas-induced mutations and pleiotropic effects of scarlet gene in an emerging model system.Heredity · 2025Article
- Article
- Mesenchymal stem cells lineage and their role in disease development.Molecular medicine (Cambridge, Mass.) · 2024Review
- Spatiotemporal control of transgene expression using an infrared laser in the crustacean Daphnia magna.Scientific reports · 2024Article
- Efficient CRISPR genome editing and integrative genomic analyses reveal the mosaicism of Cas-induced mutations and pleiotropic effects ofbioRxiv : the preprint server for biology · 2024Article
- The Transcriptomic Signature of Cyclical Parthenogenesis.Genome biology and evolution · 2023Article
- CELF1 represses Doublesex1 expression via its 5' UTR in the crustacean Daphnia magna.PloS one · 2022Article
- Article
- Production of genome-edited Daphnia for heavy metal detection by fluorescence.Scientific reports · 2020Article
- Experimental duplication of bilaterian body axes in spider embryos: Holm's organizer and self-regulation of embryonic fields.Development genes and evolution · 2020Review
- Roles of and cross-talk between ecdysteroid and sesquiterpenoid pathways in embryogenesis of branchiopod crustacean Daphnia magna.PloS one · 2020Article
- Two Doublesex1 mutants revealed a tunable gene network underlying intersexuality in Daphnia magna.PloS one · 2020Article
- Article
- Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna.PloS one · 2018Article
- Sensory Ecology of Predator-Induced Phenotypic Plasticity.Frontiers in behavioral neuroscience · 2018Review
- Mapping the expression of the sex determining factor Doublesex1 in Daphnia magna using a knock-in reporter.Scientific reports · 2017Article
- Co-option of the bZIP transcription factor Vrille as the activator of Doublesex1 in environmental sex determination of the crustacean Daphnia magna.PLoS genetics · 2017Article
- Population Genomics ofGenetics · 2017Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The water flea, Daphnia, has been the subject of study in ecology, evolution, and environmental sciences for decades. Over the last few years, expressed sequence tags and a genome sequence have been determined. In addition, functional approaches of overexpression and gene silencing based on microinjection of RNAs into eggs have been established. However, the transient nature of these approaches prevents us from analyzing gene functions in later stages of development. To overcome this limitation, transgenesis would become a key tool. Here we report establishment of a transgenic line using microinjection of plasmid into Daphnia magna eggs. The green fluorescent protein (GFP) gene fused with the D. magna histone H2B gene under the control of a promoter/enhancer region of the elongation factor 1α-1 (EF1α-1) gene, EF1α-1::H2B-GFP, was used as a reporter providing high resolution visualization of active chromatin. Transgenic lines were obtained from 0.67% of the total fertile adults that survived the injections. One of the transgenic animals, which exhibited fluorescence in the nuclei of cells during embryogenesis and oogenesis, had two copies of EF1α-1::H2B-GFP in a head-to-tail array. This is the first report of a transgenesis technique in Daphnia and, together with emerging genome sequences, will be useful for advancing knowledge of the molecular biology of Daphnia.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.