ArticlePloS one2012
High resolution melt analysis (HRMA); a viable alternative to agarose gel electrophoresis for mouse genotyping.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 39 citations in OpenAlex.
- The Evolution of Precision Anti-Doping From UGT2B17 Polymorphism Insights to Integrated Detection Frameworks.Drug testing and analysis · 2026Review
- Establishing a Mouse Genotyping Core Facility Based on Automation, High-resolution Melting Analysis, and Purpose-developed MATLAB Applications for Sample Tracking, Data Analysis, and Report Generation.Comparative medicine · 2024Article
- In-silico prediction of RT-qPCR-high resolution melting for broad detection of emaraviruses.PloS one · 2023Article
- One-Step Genotyping Method in loxP-Based Conditional Knockout Mice Generated by CRISPR-Cas9 Technology.Molecular biotechnology · 2022Article
- Genotyping and Multivariate Regression Trees Reveal Ecological Diversification within the Microcystis aeruginosa Complex along a Wide Environmental Gradient.Applied and environmental microbiology · 2022Article
- A Simple, Robust, and Cost-effective Method for Genotyping Small-scale Mutations.Journal of clinical and translational pathology · 2022Article
- SUMOylation Potentiates ZIC Protein Activity to Influence Murine Neural Crest Cell Specification.International journal of molecular sciences · 2021Article
- High-throughput genotyping of high-homology mutant mouse strains by next-generation sequencing.Methods (San Diego, Calif.) · 2021Article
- Evaluation of high-resolution melt curve analysis for rapid differentiation of Campylobacter hepaticus from other species in birds.PloS one · 2021Article
- A zebrafish functional genomics model to investigate the role of human A20 variants in vivo.Scientific reports · 2020Article
- High resolution melting based method for rapid discriminatory diagnosis of co-infecting Leptomonas seymouri in Leishmania donovani-induced leishmaniasis.Parasitology international · 2020Article
- The oncoprotein TBX3 is controlling severity in experimental arthritis.Arthritis research & therapy · 2019Article
- Swift Large-scale Examination of Directed Genome Editing.PloS one · 2019Article
- Analysis of Polymorphisms in the Mediator Complex Subunit 13-like (Med13L) Gene in the Context of Immune Function and Development of Experimental Arthritis.Archivum immunologiae et therapiae experimentalis · 2018Article
- Primate genotyping via high resolution melt analysis: rapid and reliable identification of color vision status in wild lemurs.Primates; journal of primatology · 2016Article
- Advantages and limitations of potential methods for the analysis of bacteria in milk: a review.Journal of food science and technology · 2016Review
- Identification of six New World Leishmania species through the implementation of a High-Resolution Melting (HRM) genotyping assay.Parasites & vectors · 2014Article
- The role of Zic genes in inner ear development in the mouse: Exploring mutant mouse phenotypes.Developmental dynamics : an official publication of the American Association of Anatomists · 2014Article
- Rapid and efficient zebrafish genotyping using PCR with high-resolution melt analysis.Journal of visualized experiments : JoVE · 2014Article
- A murine Zic3 transcript with a premature termination codon evades nonsense-mediated decay during axis formation.Disease models & mechanisms · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Most mouse genetics laboratories maintain mouse strains that require genotyping in order to identify the genetically modified animals. The plethora of mutagenesis strategies and publicly available mouse alleles means that any one laboratory may maintain alleles with random or targeted insertions of orthologous or unrelated sequences as well as random or targeted deletions and point mutants. Many experiments require that different strains be cross bred conferring the need to genotype progeny at more than one locus. In contrast to the range of new technologies for mouse mutagenesis, genotyping methods have remained relatively static with alleles typically discriminated by agarose gel electrophoresis of PCR products. This requires a large amount of researcher time. Additionally it is susceptible to contamination of future genotyping experiments because it requires that tubes containing PCR products be opened for analysis. Progress has been made with the genotyping of mouse point mutants because a range of new high-throughput techniques have been developed for the detection of Single Nucleotide Polymorphisms. Some of these techniques are suitable for genotyping point mutants but do not detect insertion or deletion alleles. Ideally, mouse genetics laboratories would use a single, high-throughput platform that enables closed-tube analysis to genotype the entire range of possible insertion and deletion alleles and point mutants. Here we show that High Resolution Melt Analysis meets these criteria, it is suitable for closed-tube genotyping of all allele types and current genotyping assays can be converted to this technology with little or no effort.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.