ArticlePloS one2012
Highly efficient and specific genome editing in silkworm using custom TALENs.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed, 164 citations in OpenAlex.
- The role of BmsPLA2-4 in tissue defense against pathogens in silkworm, Bombyx mori.Insect science · 2026Article
- From Gene Knockouts to Genome Remodeling: Large DNA Fragment Deletion Technologies in Plants.Plants (Basel, Switzerland) · 2026Review
- CRISPR-Mediated Silkworm: The Oncoming Agricultural Revolutions and a Rising Model Organism.Genes · 2026Review
- CRISPR-Cas13-mediated RNA editing in the silkwormZoological research · 2024Article
- High-Throughput Screening of PAM-Flexible Cas9 Variants for Expanded Genome Editing in the Silkworm (Insects · 2024Article
- High-throughput and genome-scale targeted mutagenesis using CRISPR in a nonmodel multicellular organism,Genome research · 2024Article
- High mechanical property silk produced by transgenic silkworms expressing theFrontiers in bioengineering and biotechnology · 2024Article
- Cas9-Mediated Gene Editing Using Receptor-Mediated Ovary Transduction of Cargo (ReMOT) Control inInsects · 2023Article
- Integrated Analysis of Machine Learning and Deep Learning in Silkworm Pupae (Animals : an open access journal from MDPI · 2023Article
- An Efficient Workflow for Screening and Stabilizing CRISPR/Cas9-Mediated Mutant Lines inMethods and protocols · 2020Article
- Review
- Genome-wide CRISPR screening reveals genes essential for cell viability and resistance to abiotic and biotic stresses inGenome research · 2020Article
- An Effective Microinjection Method and TALEN-Mediated Genome Editing in Pacific Abalone.Marine biotechnology (New York, N.Y.) · 2019Article
- CRISPR/Cas9-mediated PBP1 and PBP3 mutagenesis induced significant reduction in electrophysiological response to sex pheromones in male Chilo suppressalis.Insect science · 2019Article
- Extraordinary Mechanical Properties of Composite Silk Through Hereditable Transgenic Silkworm Expressing Recombinant Major Ampullate Spidroin.Scientific reports · 2018Article
- Mass spider silk production through targeted gene replacement inProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Programmable Single and Multiplex Base-Editing inG3 (Bethesda, Md.) · 2018Article
- A review on advanced methods in plant gene targeting.Journal, genetic engineering & biotechnology · 2017Review
- Transgenic silkworms secrete the recombinant glycosylated MRJP1 protein of Chinese honeybee, Apis cerana cerana.Transgenic research · 2017Article
- Increasing the yield of middle silk gland expression system through transgenic knock-down of endogenous sericin-1.Molecular genetics and genomics : MGG · 2017Article
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Establishment of efficient genome editing tools is essential for fundamental research, genetic engineering, and gene therapy. Successful construction and application of transcription activator-like effector nucleases (TALENs) in several organisms herald an exciting new era for genome editing. We describe the production of two active TALENs and their successful application in the targeted mutagenesis of silkworm, Bombyx mori, whose genetic manipulation methods are parallel to those of Drosophila and other insects. We will also show that the simultaneous expression of two pairs of TALENs generates heritable large chromosomal deletion. Our results demonstrate that (i) TALENs can be used in silkworm and (ii) heritable large chromosomal deletions can be induced by two pairs of TALENs in whole organisms. The generation and the high frequency of TALENs-induced targeted mutagenesis in silkworm will promote the genetic modification of silkworm and other insect species.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.