ArticlePloS one2012
Gene expression and functional annotation of the human ciliary body epithelia.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Circadian rhythm of intraocular pressure.The journal of physiological sciences : JPS · 2024Review
- Does Patisiran Reduce Ocular Transthyretin Synthesis? A Pilot Study of Two Cases.Current neuropharmacology · 2023Article
- Pigment Epithelia of the Eye: Cell-Type Conversion in Regeneration and Disease.Life (Basel, Switzerland) · 2022Review
- Single-cell RNA-sequencing analysis of the ciliary epithelium and contiguous tissues in the mouse eye.Experimental eye research · 2021Article
- NOTCH Signaling Controls Ciliary Body Morphogenesis and Secretion by Directly Regulating Nectin Protein Expression.Cell reports · 2021Article
- A human cell atlas of fetal gene expression.Science (New York, N.Y.) · 2020Article
- Cell atlas of aqueous humor outflow pathways in eyes of humans and four model species provides insight into glaucoma pathogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Circadian Regulation of IOP Rhythm by Dual Pathways of Glucocorticoids and the Sympathetic Nervous System.Investigative ophthalmology & visual science · 2020Article
- Prion protein modulates iron transport in the anterior segment: Implications for ocular iron homeostasis and prion transmission.Experimental eye research · 2018Article
- Stem Cell Derived Retinal Pigment Epithelium: The Role of Pigmentation as Maturation Marker and Gene Expression Profile Comparison with Human Endogenous Retinal Pigment Epithelium.Stem cell reviews and reports · 2017Article
- Major review: Molecular genetics of primary open-angle glaucoma.Experimental eye research · 2017Review
- Comparative gene expression study and pathway analysis of the human iris- and the retinal pigment epithelium.PloS one · 2017Article
- Cross-Species Analysis of Gene Expression and Function in Prefrontal Cortex, Hippocampus and Striatum.PloS one · 2016Article
- Gene expression and functional annotation of human choroid plexus epithelium failure in Alzheimer's disease.BMC genomics · 2015Article
- Article
- Validation of endothelin B receptor antibodies reveals two distinct receptor-related bands on Western blot.Analytical biochemistry · 2015Article
- Genome-wide analysis of multi-ancestry cohorts identifies new loci influencing intraocular pressure and susceptibility to glaucoma.Nature genetics · 2014Article
- Differential gene expression in glaucoma.Cold Spring Harbor perspectives in medicine · 2014Review
- Gene expression-based comparison of the human secretory neuroepithelia of the brain choroid plexus and the ocular ciliary body: potential implications for glaucoma.Fluids and barriers of the CNS · 2014Article
- In silico analysis of the molecular machinery underlying aqueous humor production: potential implications for glaucoma.Journal of clinical bioinformatics · 2013Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeThe ciliary body (CB) of the human eye consists of the non-pigmented (NPE) and pigmented (PE) neuro-epithelia. We investigated the gene expression of NPE and PE, to shed light on the molecular mechanisms underlying the most important functions of the CB. We also developed molecular signatures for the NPE and PE and studied possible new clues for glaucoma.
methodsWe isolated NPE and PE cells from seven healthy human donor eyes using laser dissection microscopy. Next, we performed RNA isolation, amplification, labeling and hybridization against 44×k Agilent microarrays. For microarray conformations, we used a literature study, RT-PCRs, and immunohistochemical stainings. We analyzed the gene expression data with R and with the knowledge database Ingenuity.
resultsThe gene expression profiles and functional annotations of the NPE and PE were highly similar. We found that the most important functionalities of the NPE and PE were related to developmental processes, neural nature of the tissue, endocrine and metabolic signaling, and immunological functions. In total 1576 genes differed statistically significantly between NPE and PE. From these genes, at least 3 were cell-specific for the NPE and 143 for the PE. Finally, we observed high expression in the (N)PE of 35 genes previously implicated in molecular mechanisms related to glaucoma.
conclusionOur gene expression analysis suggested that the NPE and PE of the CB were quite similar. Nonetheless, cell-type specific differences were found. The molecular machineries of the human NPE and PE are involved in a range of neuro-endocrinological, developmental and immunological functions, and perhaps glaucoma.
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