ArticlePloS one2012
Involvement of microRNA-mediated gene expression regulation in the pathological development of stem canker disease in Populus trichocarpa.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers.
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Who cites it
54 citing papers in PubMed, 124 citations in OpenAlex.
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- Small RNAs: Efficient and miraculous effectors that play key roles in plant-microbe interactions.Molecular plant pathology · 2023Review
- The responses of poplars to fungal pathogens: A review of the defensive pathway.Frontiers in plant science · 2023Review
- Single-step genome-wide association study for susceptibility toFrontiers in plant science · 2023Article
- Integrated transcriptome and microRNA sequencing analyses reveal gene responses in poplar leaves infected by the novel pathogen bean common mosaic virus (BCMV).Frontiers in plant science · 2023Article
- Fungal Secondary Metabolites and Small RNAs Enhance Pathogenicity during Plant-Fungal Pathogen Interactions.Journal of fungi (Basel, Switzerland) · 2022Review
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- Recent advances and potential applications of cross-kingdom movement of miRNAs in modulating plant's disease response.RNA biology · 2022Review
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- Genotoxic Evaluation of FeMolecules (Basel, Switzerland) · 2021Article
- microRNA 166: an evolutionarily conserved stress biomarker in land plants targeting HD-ZIP family.Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Review
- Differential expression profiling of microRNAs and their target genes during wheat-Physiology and molecular biology of plants : an international journal of functional plant biology · 2021Article
- Identification and Characterization ofPlants (Basel, Switzerland) · 2021Article
- miRNA Mediated Regulation and Interaction between Plants and Pathogens.International journal of molecular sciences · 2021Review
- MicroRNAs in Woody Plants.Frontiers in plant science · 2021Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs), a type of short (21-23 nucleotides), non-coding RNA molecule, mediate repressive gene regulation through RNA silencing at the post-transcriptional level, and play an important role in defense and response to abiotic and biotic stresses. In the present study, Affymetrix® miRNA Array, real-time quantitative PCR (qPCR) for miRNAs and their targets, and miRNA promoter analysis were used to validate the gene expression patterns of miRNAs in Populus trichocarpa plantlets induced with the poplar stem canker pathogen, Botryosphaeria dothidea. Twelve miRNAs (miR156, miR159, miR160, miR164, miR166, miR168, miR172, miR319, miR398, miR408, miR1448, and miR1450) were upregulated in the stem bark of P. trichocarpa, but no downregulated miRNAs were found. Based on analysis of the miRNAs and their targets, a potential co-regulatory network was developed to describe post-transcriptional regulation in the pathological development of poplar stem canker. There was highly complex cross-talk between diverse miRNA pathway responses to biotic and abiotic stresses. The results suggest that miR156 is probably an integral component of the miRNA response to all environmental stresses in plants. Cis-regulatory elements were binding sites for the transcription factors (TFs) on DNA. Promoter analysis revealed that TC-rich repeats and a W1-box motif were both tightly related disease response motifs in Populus. Promoter analysis and target analysis of miRNAs also revealed that some TFs regulate their activation/repression. Furthermore, a feedback regulatory network in the pathological development of poplar stem canker is provided. The results confirm that miRNA pathways regulate gene expression during the pathological development of plant disease, and provide new insights into understanding the onset and development of poplar stem canker.
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