ArticlePloS one2012
The redox-sensing regulator Rex modulates central carbon metabolism, stress tolerance response and biofilm formation by Streptococcus mutans.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 62 citations in OpenAlex.
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- Systematic analysis of the glucose-PTS inApplied and environmental microbiology · 2025Article
- Cordycepin affects Streptococcus mutans biofilm and interferes with its metabolism.BMC oral health · 2025Article
- Multi-omics analysis reveals genes and metabolites involved in Streptococcus suis biofilm formation.BMC microbiology · 2024Article
- Multiple factors regulate the expression ofFrontiers in cellular and infection microbiology · 2024Article
- Two novel genes identified by large-scale transcriptomic analysis are essential for biofilm and rugose colony development of Vibrio vulnificus.PLoS pathogens · 2023Article
- Revealing the Characteristics of Glucose- and Lactate-Based Chain Elongation for Caproate Production bymSystems · 2022Article
- Adaptation to simulated microgravity in Streptococcus mutans.NPJ microgravity · 2022Article
- Disruption of theJournal of bacteriology · 2022Article
- Structural Basis of Redox-Sensing Transcriptional Repressor Rex with Cofactor NADInternational journal of molecular sciences · 2022Article
- Characterization of the RpoN regulon reveals the regulation of motility, T6SS2 and metabolism inFrontiers in microbiology · 2022Article
- Incorporation of Arginine to Commercial Orthodontic Light-Cured Resin Cements-Physical, Adhesive, and Antibacterial Properties.Materials (Basel, Switzerland) · 2021Article
- NAD+ pool depletion as a signal for the Rex regulon involved in Streptococcus agalactiae virulence.PLoS pathogens · 2021Article
- Genome-Wide Transcription Factor DNA Binding Sites and Gene Regulatory Networks inFrontiers in microbiology · 2021Article
- Transcriptome Analysis Reveals Catabolite Control Protein A Regulatory Mechanisms Underlying Glucose-Excess or -Limited Conditions in a Ruminal Bacterium,Frontiers in microbiology · 2021Article
- Reuterin disruptsGut microbes · 2020Article
- Analysis of cariogenic potential of alternative milk beverages by in vitro Streptococcus mutans biofilm model and ex vivo caries model.Archives of oral biology · 2019Article
- The Redox-Sensing Regulator Rex Contributes to the Virulence and Oxidative Stress Response ofFrontiers in cellular and infection microbiology · 2018Article
- Analysis of the Cariogenic Potential of Various Almond Milk Beverages using a Streptococcus mutans Biofilm Model in vitro.Caries research · 2018Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
The Rex repressor has been implicated in regulation of central carbon and energy metabolism in gram-positive bacteria. We have previously shown that Streptococcus mutans, the primary causative agent of dental caries, alters its transcriptome upon Rex-deficiency and renders S. mutans to have increased susceptibility to oxidative stress, aberrations in glucan production, and poor biofilm formation. In this study, we showed that rex in S. mutans is co-transcribed as an operon with downstream guaA, encoding a putative glutamine amidotransferase. Electrophoretic mobility shift assays showed that recombinant Rex bound promoters of target genes avidly and specifically, including those down-regulated in response to Rex-deficiency, and that the ability of recombinant Rex to bind to selected promoters was modulated by NADH and NAD(+). Results suggest that Rex in S. mutans can function as an activator in response to intracellular NADH/NAD(+) level, although the exact binding site for activator Rex remains unclear. Consistent with a role in oxidative stress tolerance, hydrogen peroxide challenge assays showed that the Rex-deficient mutant, TW239, and the Rex/GuaA double mutant, JB314, were more susceptible to hydrogen peroxide killing than the wildtype, UA159. Relative to UA159, JB314 displayed major defects in biofilm formation, with a decrease of more than 50-fold in biomass after 48-hours. Collectively, these results further suggest that Rex in S. mutans regulates fermentation pathways, oxidative stress tolerance, and biofilm formation in response to intracellular NADH/NAD(+) level. Current effort is being directed to further investigation of the role of GuaA in S. mutans cellular physiology.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.