ArticlePloS one2012
Generation and characterization of the Western Regional Research Center Brachypodium T-DNA insertional mutant collection.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 118 citations in OpenAlex.
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- Transposition of HOPPLA in siRNA-deficient plants suggests a limited effect of the environment on retrotransposon mobility in Brachypodium distachyon.PLoS genetics · 2024Article
- Group VII ethylene response factors forming distinct regulatory loops mediate submergence responses.Plant physiology · 2024Article
- Submergence Stress Alters the Expression of Clock Genes and Configures New Zeniths and Expression of Outputs inInternational journal of molecular sciences · 2023Article
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- Loss of PROTEIN TARGETING TO STARCH 2 has variable effects on starch synthesis across organs and species.Journal of experimental botany · 2022Article
- Bacillus velezensis strain B26 modulates the inflorescence and root architecture of Brachypodium distachyon via hormone homeostasis.Scientific reports · 2022Article
- Comparative Hessian Fly Larval Transcriptomics Provides Novel Insight into Host and Nonhost Resistance.International journal of molecular sciences · 2021Article
- Stacking AsFMT overexpression with BdPMT loss of function enhances monolignol ferulate production in Brachypodium distachyon.Plant biotechnology journal · 2021Article
- Identification of Candidate Susceptibility Genes toFrontiers in plant science · 2021Article
- Global analysis of SBP gene family in Brachypodium distachyon reveals its association with spike development.Scientific reports · 2020Article
- A triticale tapetal non-specific lipid transfer protein (nsLTP) is translocated to the pollen cell wall.Plant cell reports · 2020Article
- Regulation of monocot and dicot plant development with constitutively active alleles of phytochrome B.Plant direct · 2020Article
- In Vitro Tissue Culture inInternational journal of molecular sciences · 2020Review
- Characterisation of Cellulose Synthase Like F6 (Frontiers in plant science · 2020Article
- A CRISPR/Cas9-Based Mutagenesis Protocol forFrontiers in plant science · 2020Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The model grass Brachypodium distachyon (Brachypodium) is an excellent system for studying the basic biology underlying traits relevant to the use of grasses as food, forage and energy crops. To add to the growing collection of Brachypodium resources available to plant scientists, we further optimized our Agrobacterium tumefaciens-mediated high-efficiency transformation method and generated 8,491 Brachypodium T-DNA lines. We used inverse PCR to sequence the DNA flanking the insertion sites in the mutants. Using these flanking sequence tags (FSTs) we were able to assign 7,389 FSTs from 4,402 T-DNA mutants to 5,285 specific insertion sites (ISs) in the Brachypodium genome. More than 29% of the assigned ISs are supported by multiple FSTs. T-DNA insertions span the entire genome with an average of 19.3 insertions/Mb. The distribution of T-DNA insertions is non-uniform with a larger number of insertions at the distal ends compared to the centromeric regions of the chromosomes. Insertions are correlated with genic regions, but are biased toward UTRs and non-coding regions within 1 kb of genes over exons and intron regions. More than 1,300 unique genes have been tagged in this population. Information about the Western Regional Research Center Brachypodium insertional mutant population is available on a searchable website (http://brachypodium.pw.usda.gov) designed to provide researchers with a means to order T-DNA lines with mutations in genes of interest.
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