ArticlePloS one2012
A flow cytometry-based assay for quantifying non-plaque forming strains of yellow fever virus.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 12 citations in OpenAlex.
- Evolution of the PRNT: Merging tradition and innovation to set the gold standard in the era of automation.Human vaccines & immunotherapeutics · 2025Review
- Development of a hydrogen peroxide-inactivated vaccine that protects against viscerotropic yellow fever in a non-human primate model.Cell reports. Medicine · 2024Article
- An evolutionarily conserved ubiquitin ligase drives infection and transmission of flaviviruses.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- FluoRNT: A robust, efficient assay for the detection of neutralising antibodies against yellow fever virus 17D.PloS one · 2022Article
- Consumptive coagulopathy of severe yellow fever occurs independently of hepatocellular tropism and massive hepatic injury.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Alternative pathway of complement activation has a beneficial role against Chandipura virus infection.Medical microbiology and immunology · 2020Article
- Pathophysiologic and transcriptomic analyses of viscerotropic yellow fever in a rhesus macaque model.PLoS neglected tropical diseases · 2014Article
- Advances and controversies in yellow fever vaccination.Therapeutic advances in vaccines · 2013Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Primary clinical isolates of yellow fever virus can be difficult to quantitate by standard in vitro methods because they may not form discernable plaques or induce a measurable cytopathic effect (CPE) on cell monolayers. In our hands, the Dakar strain of yellow fever virus (YFV-Dakar) could not be measured by plaque assay (PA), focus-forming assay (FFA), or by measurement of CPE. For these reasons, we developed a YFV-specific monoclonal antibody (3A8.B6) and used it to optimize a highly sensitive flow cytometry-based tissue culture limiting dilution assay (TC-LDA) to measure levels of infectious virus. The TC-LDA was performed by incubating serial dilutions of virus in replicate wells of C6/36 cells and stained intracellularly for virus with MAb 3A8.B6. Using this approach, we could reproducibly quantitate YFV-Dakar in tissue culture supernatants as well as from the serum of viremic rhesus macaques experimentally infected with YFV-Dakar. Moreover, the TC-LDA approach was >10-fold more sensitive than standard plaque assay for quantitating typical plaque-forming strains of YFV including YFV-17D and YFV-FNV (French neurotropic vaccine). Together, these results indicate that the TC-LDA technique is effective for quantitating both plaque-forming and non-plaque-forming strains of yellow fever virus, and this methodology may be readily adapted for the study and quantitation of other non-plaque-forming viruses.
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Registered trials
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