ArticlePLoS genetics2012
Co-evolution of mitochondrial tRNA import and codon usage determines translational efficiency in the green alga Chlamydomonas.
Article in PLoS genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
- Mitogenome structure and phylogenomics of the order Thoreales (Nemaliophycidae, Rhodophyta).Journal of phycology · 2026Article
- Complete mitochondrial genome of Persicaria maackiana reveals genome features, phylogenetic relationships, and nucleotide substitution rates.Scientific reports · 2025Article
- Biolistic Transformation of Chlamydomonas reinhardtii and Saccharomyces cerevisiae Mitochondria.Methods in molecular biology (Clifton, N.J.) · 2023Article
- How to build a ribosome from RNA fragments in Chlamydomonas mitochondria.Nature communications · 2021Article
- Article
- Correction: Co-Evolution of Mitochondrial tRNA Import and Codon Usage Determines Translational Efficiency in the Green Alga Chlamydomonas.PLoS genetics · 2020Article
- A Series of Fortunate Events: Introducing Chlamydomonas as a Reference Organism.The Plant cell · 2019Review
- Rapid Genetic Code Evolution in Green Algal Mitochondrial Genomes.Molecular biology and evolution · 2019Article
- Plastome-Wide Rearrangements and Gene Losses in Carnivorous Droseraceae.Genome biology and evolution · 2019Article
- Comprehensive Discovery of Cell-Cycle-Essential Pathways inThe Plant cell · 2018Article
- Aminoacyl-tRNA synthetase evolution and sectoring of the genetic code.Transcription · 2018Article
- The role of intracellular compartmentalization on tRNA processing and modification.RNA biology · 2018Review
- Polycytidylation of mitochondrial mRNAs in Chlamydomonas reinhardtii.Nucleic acids research · 2017Article
- ChloroMitoCU: Codon patterns across organelle genomes for functional genomics and evolutionary applications.DNA research : an international journal for rapid publication of reports on genes and genomes · 2017Article
- Blastocystis Mitochondrial Genomes Appear to Show Multiple Independent Gains and Losses of Start and Stop Codons.Genome biology and evolution · 2016Article
- Chimeric mitochondrial peptides from contiguous regular and swinger RNA.Computational and structural biotechnology journal · 2016Article
- tRNA biology in mitochondria.International journal of molecular sciences · 2015Review
- tRNA gene diversity in the three domains of life.Frontiers in genetics · 2014Review
- Life without tRNAArg-adenosine deaminase TadA: evolutionary consequences of decoding the four CGN codons as arginine in Mycoplasmas and other Mollicutes.Nucleic acids research · 2013Article
- Functional recurrent mutations in the human mitochondrial phylogeny: dual roles in evolution and disease.Genome biology and evolution · 2013Article
Corrections and comments
- Erratum issued
Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mitochondria from diverse phyla, including protozoa, fungi, higher plants, and humans, import tRNAs from the cytosol in order to ensure proper mitochondrial translation. Despite the broad occurrence of this process, our understanding of tRNA import mechanisms is fragmentary, and crucial questions about their regulation remain unanswered. In the unicellular green alga Chlamydomonas, a precise correlation was found between the mitochondrial codon usage and the nature and amount of imported tRNAs. This led to the hypothesis that tRNA import might be a dynamic process able to adapt to the mitochondrial genome content. By manipulating the Chlamydomonas mitochondrial genome, we introduced point mutations in order to modify its codon usage. We find that the codon usage modification results in reduced levels of mitochondrial translation as well as in subsequent decreased levels and activities of respiratory complexes. These effects are linked to the consequential limitations of the pool of tRNAs in mitochondria. This indicates that tRNA mitochondrial import cannot be rapidly regulated in response to a novel genetic context and thus does not appear to be a dynamic process. It rather suggests that the steady-state levels of imported tRNAs in mitochondria result from a co-evolutive adaptation between the tRNA import mechanism and the requirements of the mitochondrial translation machinery.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.