ArticlePLoS genetics2012
An essential role of variant histone H3.3 for ectomesenchyme potential of the cranial neural crest.
Article in PLoS genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 57 citations in OpenAlex.
- Facial Cartilage Development in Jawed Fish.Development & reproduction · 2026Review
- Neural crest gene regulatory networks as drivers of development, diversification and disease.Nature reviews. Molecular cell biology · 2026Review
- TFAP2 paralogs regulate midfacial development in part through a conserved ALX genetic pathway.Development (Cambridge, England) · 2024Article
- Reassessing the embryonic origin and potential of craniofacial ectomesenchyme.Seminars in cell & developmental biology · 2023Review
- Control of cranial ectomesenchyme fate by Nr2f nuclear receptors.Development (Cambridge, England) · 2022Article
- Ash2l, an obligatory component of H3K4 methylation complexes, regulates neural crest development.Developmental biology · 2022Article
- Postmitotic accumulation of histone variant H3.3 in new cortical neurons establishes neuronal chromatin, transcriptome, and identity.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Multi-omic profiling of histone variant H3.3 lysine 27 methylation reveals a distinct role from canonical H3 in stem cell differentiation.Molecular omics · 2022Article
- Review
- Transgenic fluorescent zebrafish lines that have revolutionized biomedical research.Laboratory animal research · 2021Review
- A comprehensive series of Irx cluster mutants reveals diverse roles in facial cartilage development.Development (Cambridge, England) · 2021Article
- Histone H3.3 beyond cancer: Germline mutations inScience advances · 2020Article
- Histones, Their Variants and Post-translational Modifications in Zebrafish Development.Frontiers in cell and developmental biology · 2020Review
- Article
- Altered bone growth dynamics prefigure craniosynostosis in a zebrafish model of Saethre-Chotzen syndrome.eLife · 2018Article
- Craniofacial genetics: Where have we been and where are we going?PLoS genetics · 2018Article
- Mllt10 knockout mouse model reveals critical role of Af10-dependent H3K79 methylation in midfacial development.Scientific reports · 2017Article
- Shifting Zebrafish Lethal Skeletal Mutant Penetrance by Progeny Testing.Journal of visualized experiments : JoVE · 2017Article
- Review
- Histone turnover and chromatin accessibility: Critical mediators of neurological development, plasticity, and disease.BioEssays : news and reviews in molecular, cellular and developmental biology · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The neural crest (NC) is a vertebrate-specific cell population that exhibits remarkable multipotency. Although derived from the neural plate border (NPB) ectoderm, cranial NC (CNC) cells contribute not only to the peripheral nervous system but also to the ectomesenchymal precursors of the head skeleton. To date, the developmental basis for such broad potential has remained elusive. Here, we show that the replacement histone H3.3 is essential during early CNC development for these cells to generate ectomesenchyme and head pigment precursors. In a forward genetic screen in zebrafish, we identified a dominant D123N mutation in h3f3a, one of five zebrafish variant histone H3.3 genes, that eliminates the CNC-derived head skeleton and a subset of pigment cells yet leaves other CNC derivatives and trunk NC intact. Analyses of nucleosome assembly indicate that mutant D123N H3.3 interferes with H3.3 nucleosomal incorporation by forming aberrant H3 homodimers. Consistent with CNC defects arising from insufficient H3.3 incorporation into chromatin, supplying exogenous wild-type H3.3 rescues head skeletal development in mutants. Surprisingly, embryo-wide expression of dominant mutant H3.3 had little effect on embryonic development outside CNC, indicating an unexpectedly specific sensitivity of CNC to defects in H3.3 incorporation. Whereas previous studies had implicated H3.3 in large-scale histone replacement events that generate totipotency during germ line development, our work has revealed an additional role of H3.3 in the broad potential of the ectoderm-derived CNC, including the ability to make the mesoderm-like ectomesenchymal precursors of the head skeleton.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.